Quantification of the Major Bile Acids in Bear Bile Powder by HPLC-ELSD
SHI Yan1,2, WEI Feng1, LIN Rui-chao2,3, MA Shuang-cheng1
1. National Institutes for Food and Drug Control, Beijing 100050, China; 2. School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University, Shenyang 110016, China; 3. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
Abstract��OBJECTIVE To establish an HPLC-ELSD method for simultaneous quantification of the major bile acids in bear bile powder in its quality assessment and control. METHODS The chromatographic separation was carried out on Agilent SB-C18 column (4.6 mm��250 mm, 5 ��m) at 40 �� using 10 mmol��L-1 ammonium acetate solution and acetonitrile as the mobile phase at a flow rate of 1.0 mL��min-1. The detection was performed using evaporative light scattering detector at drift tube temperature of 110 �� and nitrogen flow rate of 2.8 L��h-1 with 1 as the gain. RESULTS The standard curves of tauroursodeoxycholic acid and taurochenodeoxycholic acid showed good relationship in the ranges of 0.087 2-0.348 8 and 0.076 0-0.342 0 mg��mL-1, respectively. The average recoveries were 101.9% (RSD was 1.9%) and 102.2% (RSD was 1.5%), respectively. CONCLUSION The method is simple, rapid, and accurate. The analysis of the result showed that the method could be used i the quality assessment and control of bear bile powder.
ʯ��, κ��, ����, ��˫��. �ܵ�������Ҫ��֭����ɷֵIJⶨ������[J]. �й�ҩѧ��־, 2016, 51(22): 1958-1961.
SHI Yan, WEI Feng, LIN Rui-chao, MA Shuang-cheng. Quantification of the Major Bile Acids in Bear Bile Powder by HPLC-ELSD. Chinese Pharmaceutical Journal, 2016, 51(22): 1958-1961.
Ch. P (2010) Vol ��(�й�ҩ��2010���.һ��) [S]. 2010:appendix 27.
[2]
Drug Specifications Promulgated by Ministry of Public Health. The Specifications of New Drugs Becoming Official (������ҩƷ�� ��ҩת����) [S]. WS3-09(B-09)-96(Z).
[3]
XU G J, XU L S, WANG Z T, et al. Varieties Systematics and Quality Research for Commonly Used Traditional Chinese Medicine Southern The Southern Cooperative Vol ��(������ҩ��Ʒ������������о� �Ϸ�Э���� ������) [M]. Fuzhou:Fujian Scientific and Technological Press,1999:860.
[4]
XU Y C, WANG Y. The research progress of bear bile powder [J]. West China Pharm Sci (����ҩѧ��־), 2000, 1(3):200-202.
[5]
WU G L, WU X H, DONG T W, et al. The research progress of constitutes without bile acids in bear bile (powder) [J]. Acta Chin Med Pharm (��ҽҩѧ��) , 2011, 39(3):90-92.
[6]
LIU G Y, LI J, WANG X L, et al. The conjugated bile acids of man-made drainage bear bile [J]. Northwest Pharm J (����ҩѧ��־), 2011, 26(5):322-323.
[7]
ZHANG Q M, YUAN H N. The research review of bile acids in bear bile powder [J]. China J Chin Mater Med (�й���ҩ��־), 1994, 19(3):182-184.
[8]
XIE P S, LIANG G H, YAN Y Z. The test and quality inspect of bear bile-TLC comparative authentication of bile acids in commodity of bear bile [J]. Chin Pharm Anal (ҩ�������־), 1981, 1(3):137-144.
[9]
ZHANG Q M, JIANG J N, YAN K D, et al. Determination of ursodeoxycholic acid and chenodeoxycholic acid in drainage bear bile [J]. Chin Pharm Anal (ҩ�������־), 1990, 10(2):102-105.
[10]
SHI Y, ZOU Q W, WEI F, et al. The research progress of bear gall powder [J]. Chin Pharm Aff (�й�ҩ��), 2014, 28(1):82-85.
2016, Vol. 51 
