目的 探讨艾迪注射液对顺铂所致大鼠急性肾毒性的保护作用。方法 40只雄性SD大鼠随机分为5组,分别为对照组,顺铂组,艾迪低、中、高剂量组(5、10、15 mL·kg-1)。除对照组外,其他组大鼠均于第3天单次腹腔注射给予顺铂10 mg·kg-1,艾迪各剂量组分别给予艾迪注射液5、10、15 mL·kg-1连续7 d;正常组和顺铂组给予等体积生理盐水。第8天测定大鼠血清肌酐(Scr)和尿素氮(BUN)含量,检测肾脏组织超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GSH-Px)、丙二醛(MDA)水平,HE染色切片观察肾组织病理变化。结果 与对照组相比,顺铂组大鼠血清Scr、BUN和MDA含量明显上升,SOD和GSH-Px含量显著下降,病理损伤严重,肾小管上皮细胞凋亡,并出现炎症;艾迪低、中、高剂量组均能改善大鼠肾脏组织形态,艾迪低剂量组大鼠血清Scr、BUN含量显著下降,SOD和GSH-Px活性显著升高;艾迪中剂量组SOD和GSH-Px活性升高;艾迪高剂量组除了能增强GSH-Px活性外,对其他指标影响均无统计学意义。结论 艾迪低、中剂量组对顺铂所致的大鼠肾毒性均有保护作用,其作用机制可能与其抗氧化作用有关。
Abstract
OBJECTIVE To investigate the protective effects of aidi injection on cisplatin (CDDP)-induced acute nephrotoxicity in rats. METHODS Forty male Sprague-Dawley rats were randomly divided into 5 groups, including the control group, CDDP group, the low, medium and high dose Aidi injection group (5, 10, 15 mL·kg-1). Except the rats in control group, the rats of other groups were injected with CDDP on the third day. Aidi injection groups were administered to the rats once daily for 7 consecutive days. The control and CDDP groups were given corresponding regime of saline. On the eighth day, determine the content of serum creatinine (Scr) and blood urea nitrogen (BUN) in the rat, detect the level of superoxide dismutase (SOD), glutathione reductase (GSH-Px) and malondialdehyde (MDA) in the kidney tissue; observe HE staining slices of rats and find the renal pathological changes. RESULTS Compared with control group, the levels of Scr, BUN and MDA content were increased significantly in CDDP group, whereas the contents of SOD and GSH-Px were decreased significantly. There were serious, renal tubular epithelial cell apoptosis and inflammation in the kidney tissue. Kidney histopathology indicated that Aidi injection alleviated CDDP-induced tissue damage. The levels of Scr, BUN were decreased significantly in the low dose Aidi injection group, whereas the activity of SOD and GSH-Px were increased significantly. The medium dose of Aidi injection could increase the level of SOD and GSH-Px. The high dose of Aidi injection could enhance the activity of GSH-Px, without statistically significant effect on other indicators. CONCLUSION The RESULTS indicate that the low and middle dose of Aidi injection might protect the renal tissue from nephrotoxicity induced by cisplatin through anti-oxidation.
关键词
顺铂 /
艾迪注射液 /
肾毒性 /
抗氧化
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Key words
cisplatin /
Aidi injection /
nephrotoxicity /
antioxidant
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中图分类号：
R944
R965
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参考文献
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脚注
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基金
北京中医药大学研究生自主课题(2015-JYB-XS087)
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