1.Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069,China; 2. Institute of Chinese Medical Sciences, University of Macau, Macau, China; 3. Sichuan New Lotus Traditional Chinese Herb Co., Ltd. Chengdu 611731, China
Abstract��OBJECTIVE To develop a method of quantitative analysis of multi-components by single marker(QAMS) for simultaneous determination of five compounds in extract of Penthorum chinense Pursh. METHODS A HPLC method was established on an Agilent ZORBAX SB-C18 column(4.6 mm��250 mm, 5 ��m). The mobile phase was acetonitrile-0.2% formic acid aqueous solution. Gradient elution was performed at a flow rate of 1.0 mL��min-1. The column temperature was 30 �� and the detection wavelength was set at 280 nm. Pinocembrin-7-O-��-D-glucoside was selected as the internal reference substance, and the relative correlation factors(RCF) of gallic acid, brevifolin carboxylic acid, 2, 6-dihydroxyacetophenone-4-O-��-D-glucoside, quercetin-3-O-��-D-xyloside and quercetin-3'-O-��-L-rhamnoside were determined by HPLC. The contents of the six components were determined by both QAMS method and external standard method and the RESULTS were compared. The accuracy and feasibility of the new method were validated by QAMS method and external standard method. RESULTS No significant differences were observed in the determination RESULTS of five constituents except brevifolin carboxylicacid in 21 batches of extract of P. chinense by QAMS method and external standard method. CONCLUSION The QAMS method is feasible and suitable to determine the contents of five constituents in the extract of P. chinense.
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2016, Vol. 51 
