Abstract��OBJECTIVE To develop a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for determination of amikacin, dexamethasone, and ribavirin in human plasma. METHODS With kanamycin as internal standard (IS), samples involve a one-step extraction with methanol of 0.2 mL plasma. The analysis was carried out on the Inertsil HILIC (2.1 mm��150 mm,3.5 ��m) column with gradient elution at a flow rate of 0.4 mL��min-1. The mobile phase was acetonitrile (containing 0.1% formic acid) and 0.1% formic acid. The determination was performed on a triple quadrupole tandem mass spectrometer by multiple reactions monitoring (MRM) mode via electrospray ionization (ESI) source. RESULTS The standard curves were linear (r�� 0.999) over the concentration range of 1.038-1 038 ng��mL-1 (amikacin), 1.063-1 063 ng��mL-1 (dexamethasone), and 1.029-1 029 ng��mL-1(ribavirin) with the lower limit of quantification(LLOQ) of 1.038,1.063,1.029 ng��mL-1. The intra- and inter-day precision (RSD) value was below 13.7% and accuracy (relative error) was ranged from -9.2% to 10.9% at all three quality control (QC) level. CONCLUSION The method for determination of amikacin, dexamethasone, and ribavirin in human plasma is rapid sensitive and accurate, as well as suitable to evaluate the and forensic toxicological analysis.
�Ŵ�, ÷�廪, ¬����, ��׳��, �����. Һ��ɫ��-���������ⶨ��Ѫ���а����ǡ��������ɺ�����Τ�ֵ�Ũ��[J]. �й�ҩѧ��־, 2016, 51(11): 920-924.
ZHANG Cong, MEI Qing-hua, LU Hui-qin, LIN Zhuang-min, LIANG Jin-cheng. Quantitative Determination of Amikacin Dexamethasone and Ribavirin in Human Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry. Chinese Pharmaceutical Journal, 2016, 51(11): 920-924.
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2016, Vol. 51 
