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�й�ҩѧ��־ 2014, Vol. 49 Issue (13) :1156-1160    DOI: 10.11669/cpj.2014.13.014
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CHEN Wei-jun1, ZHI Yong-gang1, JIANG Xiao-rui 2, WU Zhi-yuan3, LI Guo-chang1 *
1.Department of Pharmacy,The First Affiliated Hospital of the Medical College of Shihezi University, Shihezi 832008, China;
2.Shihezi Health School Xinjiang Shihezi 832000, China;
3.Shihezi Normal School Xinjiang Shihezi 832000, China

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ժҪ Ŀ�� ̽��������������-��-1031C/T��-863A/C��-857C/T��-308A/Gλ������̬���ں����ά������IJ��켰��������������-���������Ĺ��������� ���þۺ�ø����Ӧ��ֱ�ӻ�����򷨱Ƚ�121������ 126ά����彡����Ⱥ�����͡���λ����Ƶ�ʺ͵�����Ƶ�ʵIJ��졣ELISA���ⶨ��������ȺѪ��������������-������ˮƽ����� ������������-������-1031C/Tλ��CC��CT��TT������Ƶ�ʷֱ�Ϊ����(4.96%��35.54%��59.50%)��ά�����(3.17%��27.78%��69.05%);-863A/Cλ��AA��AC��CC������Ƶ�ʷֱ�Ϊ����(4.13%��28.93%��66.94%)��ά�����(0.79%��23.02%��76.19%); -857C/Tλ��CT��TT��CC������Ƶ�ʷֱ�Ϊ����(26.45%��0.00%�� 73.55%)��ά�����(27.78%��2.38%��69.84%);-308A/Gλ�� AA��AG��GG ������Ƶ�ʷֱ�Ϊ����(0.83%��9.09%�� 90.08%)��ά�����(2.38%��17.46%��80.16%);����������TCCAΪ�߷����ͣ�CCCAΪ�ͷ����͡����� Ѫ��������������-���ķ���ˮƽ�����������ڲ��첢������������Ӱ��;������������-��-308A/Gλ�������Ƶ�ʺ͵�λ����Ƶ���ں����ά�������Ⱥ��ķֲ����������Բ��죬��ʾ�½�ά�������Ⱥ����ֲ��뺺����Ⱥ��ͬ���ص㡣
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Abstract�� OBJECTIVE To investigate the TNF-�� gene promoter TNF-��-1031C/T��-863A/C��-857C/T��-308A/G polymorphism in difference between Han and Uygur, while gene polymorphism relationship with TNF-�� contents. METHODS Using polymerase chain reaction and direct gene sequencing compare 121 cases of Han and 126 cases of Uygur healthy people the differences in genotype, allele frequency and haplotype frequencies. Two ethnic populations of serum TNF-�� contents were measured by ELISA. RESULTS The CC, CT, TT frequency of TNF-��-1031 loci are Han (4.96%,35.54%,59.50%) and Uygur(3.17%,27.78%,69.05%) respectively; the AA,AC,CC frequency of TNF-��-863 loci are Han (4.13%,28.93%,66.94%) and Uygur (0.79%,23.02%,76.19%) respectively; the CT��TT��CC frequency of TNF-��-857 loci are Han (26.45%,0.00%, 73.55%) and Uygur (27.78%,2.38%,69.84%) respectively; the AA��AG��GG frequency of TNF-��-308 loci are Han (0.83%,9.09%,90.08%) and Uygur (2.38%,17.46%,80.16%) respectively; TCCA is high-producer-type, CCCA is low-producer-type. CONCLUSION The level of TNF-�� in serum was significant difference in Han and Uygur, it can be regulated by genetics. TNF-�� gene in loci of -308A/G has significant differences in genotypes and alleles frequency in Han and Uygur, show the characteristics of gene distribution in Xinjiang Uygur population.
Keywords�� TNF-��,   gene polymorphism,   Han ,   Uygur     
�ո�����: 2014-09-03;
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������, ���¸�, �������� .�½�ά����塢������ȺTNF-�������̬�����������������о�[J]  �й�ҩѧ��־, 2014,V49(13): 1156-1160
CHEN Wei-Jun-, ZHI Yong-Gang-, JIANG Xiao-Rui- etc .TNF-�� Gene Polymorphisms and Contents Relationship Between Han and Uygur in Xinjiang[J]  Chinese Pharmaceutical Journal, 2014,V49(13): 1156-1160
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