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�й�ҩѧ��־ 2014, Vol. 49 Issue (6) :490-495    DOI: 10.11669/cpj.2014.06.013
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1.�Ͼ���ҽҩ��ѧҩѧԺ, �Ͼ���ҽҩ��ѧ��ҩѧһ��ѧ��, �Ͼ� 210029;
2. ����ʡ��ҩ��Ч��ҩϵͳ���̼����о�����, �Ͼ� 210046;
3.������ҽѧԺ�ִ���ҩ�Ƽ��������ص�ʵ����, �ϲ� 330004
LI Jun-song1, 2, YANG Xin-wen1, PENG Wen-wen1, PAN Yang1*, CHEN Li-hua3, CAI Bao-chang1*
1. Pharmaceutical Department, Nanjing University of Treditional Chinese Medicine, National First-Class Key Discipline for Traditional Chinese Medicine of Nanjing University of Chinese Medicine, Nanjing 210029, China;2. Jiangsu Provincial TCM Engineering Technology Research Center of High Efficient Drug Delivery System (DDS, Nanjing 210046, China; 3.Key Laboratory of Modern Preparation of Treditional Chinese Medicine, Ministry of Education, Jiangxi University of Treditional Chinese Medicine, Nanchang 330004, China

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Abstract�� OBJECTIVE To investigate the intestinal absorption behaviors of three main active components, liensinine, isoliensinine and neferine in Plumula Nelumbinis extracts in intestine of rats. METHODS With phenol red as the indicator, in situ single pass intestinal perfusion (SPIP) model was used and the concentrations of the alkaloids in perfusion solution in different intestinal segments (duodenum, jejunum, ileum, and colon) were determined by HPLC. RESULTS Liensinine, isoliensinine and neferine were absorbed in whole intestinal segments with saturation phenomena. The absorption rate constant (Ka) and/or effective permeability values (Peff) of the three alkaloids mostly had significant difference (P��0.05) at middle and high concentrations of perfusion solution vs those at low concentrations. Liensinine, isoliensinine and neferine possessed better absorption in ileum and colon of intestine. The absorption in duodenum and jejunum mostly displayed no significant difference (P��0.05). In middle concentration group (117.3 ��g��mL-1), neferine was absorbed best among the three alkaloids in colon; in other intestinal sections, the Ka and Peff of the three alkaloids were sequenced as follows: neferine��liensinine��isoliensinine.CONCLUSION The transport mechanism of liensinine, isoliensinine and neferine in vivo may be active transport or facilitated diffusion.
Keywords�� Plumula Nelumbinis,   liensiniue,   isolieusinine,   neferine,   absorption,   in situ single-pass intestine perfusion,   HPLC     
�ո�����: 2013-06-11;
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�о�����:��ҩ���\�� Tel:(025)86798185 E-mail:y.pan2006@163.com;�̱���, ��, ����, ��ʿ�о�����ʦ �о�����:��ҩ����Tel:(025)85811112 E-mail:bccai@126.com     Email: y.pan2006@163.com
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���, , �������� .�������������ɷֵĴ������嵥�����������[J]  �й�ҩѧ��־, 2014,V49(6): 490-495
LI Jun-Song-, , YANG Xin-Wen- etc .Intestinal Absorption of Three Alkaloids in Plumula Nelumbinis by Rat Single-Pass Perfusion in situ Model[J]  Chinese Pharmaceutical Journal, 2014,V49(6): 490-495
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[1] FAGERHOLM U, JOHANSSON M, LENNERNAS H. Comparison between permeability coefficients in rat and human jejunum . Pharm Res, 1996, 13(9): 336-442. [2] ZAKERI-MILANI P, VALIZADEH H, TAJERZADEH H, et al. Predicting human intestinal permeability using single-pass intestinal perfusion in rat . J Pharm Pharm Sci, 2007, 10(3): 368-379. [3] HU X M. Chinese Materia: Vol 3 (�л�����:������) . Shanghai: Scientific and Technical Press, 1999: 403. [4] LIAO L, SHU Z, LI XR, et al. Research advance in chemical composition and pharmacological actions of medicinal herbs related to lotus. Shanghai J Tradit Chin Med (�Ϻ���ҽҩ��־), 2010, 44(12):82-84. [5] XU L. Studies of pharmacokinetics and biophamaceutics for liensinine . Shenyang: Shenyang Pharmaceutical University, 2001. [6] XU L, WANG S J, CHEN J M, et al. The pharmacokinetic research on liensinine in rats. J Shenyang Pharm Univ (����ҩ�ƴ�ѧѧ��), 2001, 18: 244-247. [7] HU X M, ZHANG X Z, CAI H S, et al. Study on pharmacokinetics of neferine in rabbit. Chin J Hosp Pharm (�й�ҽԺҩѧ��־), 1993, 13(3):105-107. [8] HAN L. Current situation and consideration of pharmacokinetic study on multiple components of traditional Chinese medicine. China J Chin Mater Med (�й���ҩ��־), 2008, 33(21):2442-2448. [9] DU Q Q, WANG Z G, LIU F, et al. Interaction of baicalin with berberine for intestinal absorption in rats. J Chin Pharm (�й�ҩ��), 2010, 21(11):965-967. CHAN��C G, DONG L. Research progress on extraction and separation of liensinine. J Chem Ind Eng(��ѧ��ҵ�빤�̼���), 2008, 29(2):21-25. LI K J. Study on oral absorption mechanism Indirubin. Chengdu:Sichuan University, 2007. ZANG H M. In situ rats single pass perfusion intestinal absorption of cefalexin by mass balance analysis. Pharm Clin Res (ҩѧ���ٴ��о�), 2008, 16(6):450-453. YANG Y, QI A L, PANG G X. Study on absorption kinetics of periplocin rat��s intestines. Qilu Pharm Aff(��³ҩ��), 2009, 28(2):105-107. CHEN X M, LI J S, LI W, et al. Intestinal absorption of the effective components of Schisandra chinensis Baill. by rats single-pass perfusion in situ. Acta Pharm Sin (ҩѧѧ��), 2010, 45(5):652-658. LIU R, LIU Z D, ZHANG B L, et al. In situ intestinal absorption of salvianolic acid B in rats. Chin J New Drugs (�й���ҩ��־), 2008, 17(10):852-860. YOU J, LI Q P, YU Y W, et al. Absorption of zedoary oil in rat intestine using in situ single pass perfusion model. Acta Pharm Sin (ҩѧѧ��), 2004, 39(10):849-853. DU Q, DI L Q, SHAN J J, et al. Intestinal absorption of daphnetin by rats single pass perfusion in situ. Acta Pharm Sin (ҩѧѧ��), 2009, 44(8):922-926. WANG J L, HU X M, YIN W H, et al. Study on alkloids from plumula nelumbinis. China J Chin Mater Med (�й���ҩ��־), 1991, 16(11):672-675.
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