目的 建立大鼠尿中咖啡酸、绿原酸、1，3-二咖啡酰奎宁酸浓度的高效液相色谱测定方法，研究3个化合物在大鼠尿中的排泄情况。方法 给大鼠静脉注射灯盏细辛注射液后，于不同时间段采集尿样，尿样中加入内标物原儿茶醛，经乙酸乙酯处理后，样品进HPLC分析。结果 尿样中咖啡酸、绿原酸、1，3-二咖啡酰奎宁酸的方法回收率分别约为95%～98%、90%～95%、95%～97%，60 h尿中咖啡酸、绿原酸、1，3-二咖啡酰奎宁酸的累积排泄量占给药剂量的(70.97±7.31)%、(87.62±12.23)%、(9.87±2.63)%。日内及日间精密度均小于10%。结论 该方法简便、准确、专属性强，可用于大鼠尿液中3个化合物的含量测定。
Abstract
OBJECTIVE To develop a HPLC method for the determination of the concentration of caffeic acid, chlorogenic acid and 1,3-dicaffeoylquinic acid in rat urine and to observe the excretion of three compounds in rats.METHODS Rat urine samples, collected at different intervals after vein injection of Dengzhanxixin injection traditional Chinese medicines, added with protocatechuic aldehyde as internal standard, handled by ethyl acetate, then were measured by HPLC.RESULTS The recoveries of caffeic acid, chlorogenic acid and 1,3-dicaffeoylquinic acid were about 95%-98%, 90%-95%, 95%-97%, respectively. The cumulative excretions of caffeic acid, chlorogenic acid and 1,3-dicaffeoylquinic acid in urine within 60 h were (70.97?7.31)%, (87.62?12.23)% and (9.87?2.63)%. Relative standard deviation(RSD)of intra- and inter-day assay was less than 10%.CONCLUSION The established method is simple, accurate and specific. It can be successfully used for the analysis of three compounds in rat urine.
关键词
灯盏细辛注射液 /
绿原酸 /
咖啡酸 /
1 /
3-二咖啡酰奎宁酸 /
尿 /
酚酸 /
高效液相色谱法
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Key words
Dengzhanxixin injection /
chlorogenic acid /
caffeic acid /
1,3-dicaffeoylquinic acid /
urine /
phenolic acid /
HPLC
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中图分类号：
R285
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参考文献
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脚注
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基金
科技部重大新药创制科技重大专项(2012ZX09303009-002)；江苏省中医药领军人才(LJ200906)；江苏高校优势学科建设工程资助项目(2010)
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