YANG Guang-ming1, YAN Ke1, GU Qing1, LI Jun-song1, PAN Yang1,2*, CAI Bao-chang1*
1.Key Laboratory of State Administration of Traditional Chinese Medicine for Standardization of Chinese Medicine Processing & Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China; 2.Laboratory of Medical Fungi and Phyto-Biotech, Nanjing University of Chinese Medicine, Nanjing 210023, China
Abstract��
OBJECTIVE To probe the influence of Oxytropis falcata on apoptosis and expression of Bcl-2 and Bax proteins in SMMC-7721 cells. METHODS Annexin V-FITC/ PI assay was applied to detect apoptosis in SMMC-7721 cells after being treated with TFOF (total flavonoids of O. falcata). Changes of Bcl-2 and Bax expression were investigated by Western blot. RESULTS Cell apoptosis was induced significantly in SMMC-7721 cells when subjected to TFOF. Western blot showed that Bcl-2 expression was downregulated. Although Bax expression was also downregulated slightly comparing with that of control, the ratio of Bcl-2/Bax decreased significantly after TFOF treatment. CONCLUSION The results suggest that TFOF of O. falcata may be a pro-apoptotic agent for hepatic cancer cells via its modulation of Bcl-2/Bax balance.
YANG Guang-Ming-,
YAN Ke-,
GU Qing- etc
.Effect of Tibetan Medicine Oxytropis falcata on Cell Apoptosis and Expression of Bcl-2 and Bax Protein in SMMC-7721 Cells[J] Chinese Pharmaceutical Journal, 2013,V48(24): 2113-2116
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2013, Vol. 48 
