中国药学杂志
    
           首页  |  期刊介绍  |  编 委 会  |  投稿指南  |  期刊订阅  |  广告服务  |  会议信息  |  联系我们  | 
�й�ҩѧ��־ 2013, Vol. 48 Issue (24) :2102-2106    DOI: 10.11669/cpj.2013.24.007
���� ����Ŀ¼ | ����Ŀ¼ | ������� | �߼����� << | >>
��ӥצ����Ҷ��֯��������ķֲ���rDNAITS����ϵͳ��������
����ϼ1������2����ΰƽ1���Ϻ���1����ƻ�2��������2*
1.�㶫ҩѧԺ������510006��
2.�㶫ʡ΢�����о������㶫ʡ���ֱ�����Ӧ���ص�ʵ���ң��㶫ʡ΢����Ӧ���¼�������ʵ���ң��㶫ʡ����Ӧ��΢�����ص�ʵ����-ʡ�����������ص�ʵ�����������أ�����510070
GAO Xiao-xia1, WANG Lei2, ZHOU Wei-ping1, YAN Han-jing1, LI Hao-hua2, ZHANG Wei-min2*
1.Guangdong Pharmaceutical University, Guangzhou 510006, China;
2. State Key Laboratory of Applied Microbiology Ministry��Guangdong Province Jointly Breeding Base, South China, Guangdong Open Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China

Download: PDF (0KB)   HTML (1KB)   Export: BibTeX or EndNote (RIS)      Supporting Info
ժҪ Ŀ�� �о���ӥצ(Desmos chinensis Lour.)����Ҷ��֯����������ķֲ�����rDNA ITS����ϵͳ���������� ����ʯ��������Ƭ���۲�ӥצ����Ҷ��֯��΢�ṹ��ϸ����ѧ��ȷ����������ķֲ���ƽ����뷨������������������Է���������rDNA ITS��PCR�����Ͳ���ͨ��rDNA ITS���н�����������ķ��������ϵͳ������������� ��ӥצ���е����������Ҫ�ֲ�����Ƥ������ϸ����Ҷ����Ҫ�ֲ������Ϸ����ϸ�����ڵı���ϸ����դ����֯��������֯��������14������������������⾥��ù��(Phomopsis)����������(Colletotrichum)����������(Guignardia)�������⾥��ù(P. spp.)ռ�ܾ�������57.1%�����Ϊ���ߴ�����(C. gloeosporioides)��â��������(G. mangiferae)������ ��������ڼ�ӥצ����Ҷ��֯�о��зֲ�����������֯������;rDNA ITS����ϵͳ�����ϺõĽ�ʾ�˼�ӥצ���������ϵͳ������ϵ��
Service
�ѱ����Ƽ�������
�����ҵ����
�������ù�����
Email Alert
RSS
�����������
����ϼ
����
��ΰƽ
�Ϻ���
��ƻ�
������*
�ؼ����� ��ӥצ   �������   ϵͳ����   rDNA ITS     
Abstract�� OBJECTIVE To study the distribution and phylogenesis of endophytic fungi in the stems and leaves of Desmos chinensis.METHODS Permanent paraffin-cut section, optical microscope photography, and histochemistry were used to observe the distribution of endophytic fungi in the stems and leaves of Desmos chinensis. Plate isolation method was used to separate and cultivate the endophytic fungi. The genomic DNA of endophytic fungi were extracted, then the rDNA ITS region was amplified using polymerase chain reaction (PCR). The PCR products were digested by Hae III and Hha I restriction enzymes to determine the species and genotypes, and sequenced according to the digestion genotyping results. Nucleotide sequences of the rDNA ITS of endophytic fungi were used for the identification and phylogenetic analysis. RESULTS Endophytic fungi mainly existed in the parenchyma cells of the phloem in the stems, and in the parenchyma cells, palisade tissue or sponge tissue of the leaves. Fourteen strains of endophytic fungi were isolated from the stems and leaves. Four different genotypes and their sequences were obtained from RFLP analysis and sequencing. These strains were identified as Guignardia mangiferae, Colletotrichum gloeosporioides, and Phomopsis spp. The dominant group of those endophytic strains was Phomopsis, represented by 8 strains and accounting for 57.1% of all. CONCLUSION The endophytic fungi distribute in both stems and leaves of Desmos chinensis without obvious tissue specificity. The result of the phylogenetic analyses with rDNA ITS sequences reveals the phylogenetic relationships of endophytic fungi in Desmos chinensis.
Keywords�� Desmos chinensis,   endophytic fungi,   phylogenetic analysis,   rDNA ITS     
�ո�����: 2014-01-10;
��������:�㶫ʡ��Ȼ��ѧ����������Ŀ(S2012010009773)
ͨѶ���� �������У���ʿ���о�Ա �о�����:΢������Դ����������     Email: wmzhang58@qq.com
���߼��: ����ϼ��Ů����ʿ�������� �о�������ҩ�Ƽ���������
���ñ���:   
����ϼ, ����, ��ΰƽ�� .��ӥצ����Ҷ��֯��������ķֲ���rDNAITS����ϵͳ��������[J]  �й�ҩѧ��־, 2013,V48(24): 2102-2106
GAO Xiao-Xia-, WANG Lei-, ZHOU Wei-Ping- etc .Distribution and Phylogenetic Analysis of Endophytic Fungi in Stems and Leaves of Desmos chinensis Based on Histochemistry and rDNA ITS Sequence[J]  Chinese Pharmaceutical Journal, 2013,V48(24): 2102-2106
��
[1] Editorial Committee of Flora of China of Chinese Academy of Sciense. Flora of China (�й�ֲ��־) . Beijing Science Press, 1979: 54-54.[2] JU J H, YU J G. Studies on chemical constituents of seeds of Desmos chinensis Lour.. China J Chin Mater Med (�й���ҩ��־), 1999, 24(7): 418-421.[3] WU J H, WANG X H, YI Y H, et al. Anti-AIDS agents 54: A potent anti-HIV chalcone and flavonoids from genus Desmos. Bioorg Med Chem Lett, 2003, 13(10): 1813-1815.[4] ZHENG S Q, WU J H, LIAO S X, et al. Volatile oils of Desmos Lour: A comparative studie. Chin Pharm J(�й�ҩѧ��־), 1998, 33(8): 461-464.[5] XIE H G, ZOU Z M, XU L Z. The chemical constituents and pharmacological activities of the Desmos Lour.. Drugs Clinic J (�ִ�ҩ�����ٴ�), 2006, 21(1): 14-16.[6] WU J H, LIAO S X, MAO S L, et al. Desmosflavanone��: A new flavanone from Desmos cochinchinensis Lour.. J Chin Pharm Sci, 1997, 6(3): 119-121.[7] ANDREWS J H, HIRANO S S. Microbial ecology of leaves. New York:Springer-Verlag,1991:179-197.[8] STROBEL G, YANG X S, SEARS J, et al. Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana. Microbiology, 1996, 142: 435-440.[9] WHITE T J, BRUNS T, LEE J, et al. Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for phylogenetics. In: INNIS M A, GELFAMD H, SNINSKY J J, et al, eds. PCR protocols: A guide to methods and amplifications. New York: Academic Press, 1990: 315-322. THOMPSON J D, GIBSON T J, PLEWNIAK F, et al. The CLUSTAL X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 1997, 25(24): 4876-4882. BENSON D A, KARSCH-MIZRACHI I, LIPMAN D J, et al. GenBank. Nucl Acids Res, 2003, 33(1): 34-38. SWOFFORD D L. PAUP Version 4. Sunderland, Massachusetts, Sinauer Associates, 2000. FITCH W M. Toward defining the course of evolution: Minimum change for a specific tree topology. Syst Zool, 1971, 20(4): 406-416. PATRIQUIN D G, DOBEREINER J. Light microscopy observations of tetrazolium-reducing bacteria in the endorhizosphere of maize and other grasses in Brazil. Can J Microbiol, 1978, 24(6): 734-742. BACON C W, HINTON D M. Isolation and Culture of Endophytic Bacteria and Fungi. Washington D C: ASM Press, 2007: 413-421. HORN W S, SIMMONDS M S J, SCHWARTZ R E, et al. Phomopsichalasin, a novel antimicrobial agent from an endophytic Phomopsis sp.. Tetrahedron, 1995, 51(14): 3969-3978. SILVA G H, TELES H L, ZANARDI L M, et al. Cadinane sesquiterpenoids of Phomopsis cassiae, an endophytic fungus associated with Cassia spectabilis (Leguminosae). Phytochemistry, 2006, 67(17): 1964-1969. HUANG Z J, CAI X L, SHAO C L, et al. Chemistry and weak antimicrobial activities of phomopsins produced by mangrove endophytic fungus Phomopsis sp. ZSU-H76. Phytochemistry, 2008, 69(7): 1604-1608. WU S H, CHEN Y W, SHAO S C, et al. Ten-membered lactones from Phomopsis sp., an endophytic fungus of Azadirachta indica. J Nat Prod, 2008, 71(4): 731-734. REN Y H, STROBEL G A, GRAFF J C, et al. Colutellin A, an immunosuppressive peptide from Colletotrichum dematium. Microbiology, 2008, 154(7): 1973-1979. WANG M X, CHEN S L, YAN S Z, et al. A preliminary study on the isolation, identification and media of an endophytic fungus producing flavones. J Nanjin Normal Univ(�Ͼ�ʦ����ѧѧ��), 2003, 26(1):106-110. LU H, ZOU W X, MENG J C, et al. New bioactive metabolites produced by Colletotrichum sp., an endophytic fungus in Artemisia annua. Plant Sci, 2000, 151(1): 67-73. YUAN W H, LIU M, JIANG N, et al. Guignardones A-C: Three meroterpenes from Guignardia mangiferae. Eur J Org Chem, 2010(33): 6348-6353. LIANG F L, LI D L, CHEN Y C, et al. Secondary metabolites of endophytic Guignardia mangiferae from Smilax glabra and their antitumor activities. Chin Tradit Herb Drugs (�в�ҩ), 2012, 43(5): 856-860.
[1] ��ά, ������, *, �ź��, .��ҩ�ڹ������������ķ���������־������о�[J]. �й�ҩѧ��־, 2013,48(4): 262-266
[2] �������������ź��.����ɼ�����������������俹�������о�[J]. �й�ҩѧ��־, 2013,48(3): 165-168
[3] Ԭ��,������,�����.��������ľ�������Alternaria sp.Ly81�еĻ�����໯������о�[J]. �й�ҩѧ��־, 2013,48(21): 1823-1826
[4] �¾���̷С������ӽ÷����˳��.ʯ����ֲ���������������������ֶ������о���չ[J]. �й�ҩѧ��־, 2013,48(19): 1649-1653
[5] ����������ǿ����������˳��.��Ƥʯ���������������[J]. �й�ҩѧ��־, 2013,48(19): 1669-1672
[6] �����ͣ��������Ŵ�Ϊ������������˳��.��ľ������������Ͳ�������HIV-1����ø����ɸѡ[J]. �й�ҩѧ��־, 2013,48(16): 1352-1358
[7] ������,,������,��׿,,��ѧ��,Τ����,ʷ��,��ú�,*.ëҶ��ӥצ��CB��������ĺϳ��뿹������������[J]. �й�ҩѧ��־, 2013,48(11): 924-929
[8] �����������ķ壬¬������꿣���𨣬�����º���.ֲ���������Fusariums sp.LC-1�μ���л������о�[J]. �й�ҩѧ��־, 2013,48(1): 17-21
[9] ������ ̷С�� ����÷ ��˳��.ҩ��ֲ�������������ķ��뼰���������о�[J]. �й�ҩѧ��־, 2011,46(9): 649-651
[10] ���ƻ ��Ө ����Ϊ ��� ���ٻ�.��ĵ����������ķ��뼰���־������о�[J]. �й�ҩѧ��־, 2011,46(10): 738-740
[11] ̷С�� ��˳�� ������ ����Ө.������������������ķ��뼰���������о�[J]. �й�ҩѧ��־, 2010,45(3): 178-181
[12] ������;��˳�� . 5 ��ʯ�� ��������ķ��뼰�俹�������о�[J]. �й�ҩѧ��־, 2009,44(20): 1540-1542
[13] ���ſ�;��־ϼ;��ѩ÷;������;��˳��.�ٽ������������������������ɸѡ�о�[J]. �й�ҩѧ��־, 2009,44(13): 976-979
[14] ���޺�;����c;���Ŷ�;��У��.��ݭ����������ķ��뼰�俹�����Եij����о�[J]. �й�ҩѧ��־, 2009,44(13): 972-975
[15] ��ƻ;���ø�;�⽿;���ľ�;÷����.���ϴ���������S26�д�����л������о�[J]. �й�ҩѧ��־, 2009,44(11): 825-827
Copyright 2010 by �й�ҩѧ��־