Abstract��
OBJECTIVE To study the luminescence effect of Eu3+-Metacycline (MTC)-cetyltrimethyl ammonium bromide (CTMAB) system and the luminescence mechanism of the system. METHODS Based on the luminescence effect�� a fluorescent method for the determination of MTC was proposed. Fluorescence characteristics of the complex of metacycline (MTC) with Eu3+ in a micellar system was studied�� and the effect of different concentrations of MTC and MTC complexes on Escherichia coli and the Staphylococcus aureus in optimum experimental conditions was investigated. RESULTS The experiments indicated that in pH 9.0-10.5 Na2B4O7-NaOH buffer solution�� both MTC and cationic surfactant (CTMAB) could enhance greatly the fluorescence intensity of Eu3+. The fluorescence intensity was linearly related to the fleroxacin concentration in the range of 0.45-12 ��g��mL-1. CONCLUSION A new fluorescent method has been developed for the determination of MTC in pharmaceutical preparations. The detection limit was 0.015 3 ��g��mL-1 and the RSD was 1.03%. The complexes of different concentrations of MTC and MTC have good antibacterial effect against Escherichia coli�� and the antibacterial effect is the best when the MTC��s concentration is 1��10-3 mol��L-1 against escherichia colon.
LIN Jian-Yuan,
HUANG Min-Na
.Study of Eu-Metacycline Complex Fluorescent Probe and Its Analytical Application[J] Chinese Pharmaceutical Journal, 2013,V48(2): 143-147
��
[1]
CITTERIO F. Evolution of the therapeutic drug monitoring of cyclosporine. Transplant Proc�� 2004��36(2 suppl)�� 420-425.
[2]
HUANG C B��CI Y X��YAN L�� et al. Synergistic fluorescence enhancement effect of Cd2+-Tb3+-Eu3+- deoxyteracycline triton X-100 system. Chin J Anal Chem(������ѧ)��2002��30(6)��680-683.
[3]
AO D G W��ZHANG Y��TAO Y L. Fluorescence characteristics of Mg-MTC complex in a micellar system and determination of MTC . Chin J Anal Lab(����������)��2006�� 25(2)��34-35.
[4]
WANG T. The study of interaction between rare earth ion and antibiotic drug complex and its application in life science research .Shandong Normal University(ɽ��ʦ����ѧ)��2006.
[5]
HAN J H. Interactions of northflocaxin with surfactants. Zhejiang Uinversity(�㽭��ѧ)��2006.
2013, Vol. 48 
