熟地黄提取物体外免疫调节作用实验研究

郑晓珂,侯委位,段鹏飞,冯志毅,王小兰,张艳丽,冯卫生

中国药学杂志 ›› 2012, Vol. 47 ›› Issue (24) : 1995-1999.

中国药学杂志 ›› 2012, Vol. 47 ›› Issue (24) : 1995-1999.
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熟地黄提取物体外免疫调节作用实验研究

  • 郑晓珂,侯委位,段鹏飞,冯志毅,王小兰,张艳丽,冯卫生
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Immunomodulatory Effect of Prepared Radix Rehmanniae Extract in Vitro

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摘要

目的 初步探讨熟地黄发挥免疫增强作用的活性物质及其作用机制。方法 常规分离Balb/c小鼠胸腺及脾的淋巴细胞,通过四甲基偶氮唑蓝法检测小鼠胸腺、脾淋巴细胞增殖,酶联免疫吸附法(ELISA法)检测小鼠胸腺、脾淋巴细胞上清液Th1细胞因子白细胞介素-2、干扰素-γ和Th2细胞因子白细胞介素-4、白细胞介素-5的水平。结果 熟地黄水提物(0.049、0.49、4.9 mg·mL-1)和粗多糖(0.032、0.32、3.2 mg·mL-1)均可显著促进刀豆蛋白A刺激前后小鼠胸腺及脾的淋巴细胞的增殖,提高上清液白细胞介素-2、干扰素-γ、白细胞介素-4、白细胞介素-5水平,并呈现剂量依赖性关系。结论 熟地黄具有免疫增强作用,且发挥免疫增强作用的活性物质可能为熟地黄粗多糖,作用机制与其增强T淋巴细胞Th1和Th2细胞因子的表达有关。

关键词

熟地黄 / 胸腺淋巴细胞 / 脾淋巴细胞 / 增殖 / 细胞因子 / 免疫调节

Key words

Prepared Radix Rehmanniae / thymus lymphocyte / spleen lymphocyte / proliferation / cytokine / immunomodulatory effect

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导出引用
郑晓珂,侯委位,段鹏飞,冯志毅,王小兰,张艳丽,冯卫生. 熟地黄提取物体外免疫调节作用实验研究[J]. 中国药学杂志, 2012, 47(24): 1995-1999
Immunomodulatory Effect of Prepared Radix Rehmanniae Extract in Vitro[J]. Chinese Pharmaceutical Journal, 2012, 47(24): 1995-1999
中图分类号: R965   

参考文献

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十二五国家科技支撑计划资助项目(2011BAI06B02)

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