Abstract��
OBJECTIVE To evaluate the cytotoxicity of cucurbitacin B solid lipid nanoparticles(SLN) on human neuroblastoma SK-N-SH cell line in vitro. METHODS The SK-N-SH cell line was treated with cucurbitacin B SLN at various concentrations. Growth suppression was evaluated by MTT method�� apoptosis related alterations in morphology were ascertained under light microscopy. Flow cytometry(FCM) was used to investigate the distribution of cell life. RESULTS Free cucurbitacin B and cucurbitacin B SLN inhibited the growth of SK-N-SH cells after 48 h treatment in a dose dependent manner�� with IC50 values of 0.508 and 12.6 μmol·L-1 �� respectively. The apoptotic rates at concentrations of 0.143 and 0.716 μmol·L-1 were ��34.9±4.6��% and��53.6±6.3��%�� respectively��all of which were significantly higher than that of free cucurbitacin B ��13.2±2.3��% and��43.4±5.5��% (P
XU Bing-Xin,
ZHENG Jia-Xin,
ZHAO Yuan-Yuan etc
.Cytotoxicity Evaluation of Cucurbitacin B Solid Lipid Nanoparticles (SLN) on Human Neuroblastoma SK-N-SH Cell Line in Vitro[J] Chinese Pharmaceutical Journal, 2012,V47(23): 1894-1898
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2012, Vol. 47 
