中国药学杂志
    
           首页  |  期刊介绍  |  编 委 会  |  投稿指南  |  期刊订阅  |  广告服务  |  会议信息  |  联系我们  | 
�й�ҩѧ��־ 2012, Vol. 47 Issue (23) :1894-1898    DOI:
���� ����Ŀ¼ | ����Ŀ¼ | ������� | �߼����� << | >>
��«��B����֬��������������ĸϸ����SK-N-SHϸ��������ϸ������������
�����1��2��֣����1��������1�� ��ǿ1��������2������֥1
1.֣�ݴ�ѧҩѧԺ�� ֣�� 450001��2.���������ҽԺҩѧ�������� ��� 461000
XU Bing-xin1��2�� ZHENG Jia-xin1�� TANG Yu-jie1�� ZHAO Yuan-yuan1�� LI Qiang1�� LAN Bing-xin2��WANG Yan-zhi1

Download: PDF (0KB)   HTML (1KB)   Export: BibTeX or EndNote (RIS)      Supporting Info
ժҪ Ŀ�� �о���«��B����֬��������������ĸϸ����SK-N-SHϸ��������ϸ�����ԡ����� �����ļ׻�ż��������MTT��������«��B����֬��������������ĸϸ����SK-N-SH��ϸ�����ԣ�������΢���۲�ϸ����̬����ʽϸ���Ƕ�������ϸ�����ڡ���� ����48 h�󣬺�«��B�Լ���������ʽ����SK-N-SHϸ���������� 0.143 ��0.716 μmol·L-1��«��B����֬����������SK-N-SHϸ���������ʷֱ�Ϊ��34.9±4.6��%�ͣ�53.6±6.3��%�����ӦŨ�ȵĺ�«Bԭ����ģ�13.2±2.3��%�ͣ�43.4±5.5��% ��Ƚ��������Բ���(P
Service
�ѱ����Ƽ�������
�����ҵ����
�������ù�����
Email Alert
RSS
�����������
�����
֣����
������
��ǿ
������
����֥
�ؼ����� ��«��B����֬��������   ����ĸϸ����SK-N-SHϸ��   ϸ������     
Abstract�� OBJECTIVE To evaluate the cytotoxicity of cucurbitacin B solid lipid nanoparticles(SLN) on human neuroblastoma SK-N-SH cell line in vitro. METHODS The SK-N-SH cell line was treated with cucurbitacin B SLN at various concentrations. Growth suppression was evaluated by MTT method�� apoptosis related alterations in morphology were ascertained under light microscopy. Flow cytometry(FCM) was used to investigate the distribution of cell life. RESULTS Free cucurbitacin B and cucurbitacin B SLN inhibited the growth of SK-N-SH cells after 48 h treatment in a dose dependent manner�� with IC50 values of 0.508 and 12.6 μmol·L-1 �� respectively. The apoptotic rates at concentrations of 0.143 and 0.716 μmol·L-1 were ��34.9±4.6��% and��53.6±6.3��%�� respectively��all of which were significantly higher than that of free cucurbitacin B ��13.2±2.3��% and��43.4±5.5��% (P
Keywords�� cucurbitacin B solid lipid nanoparticles,   SK-N-SH cell line,   cytotoxicity     
�ո�����: 2012-04-01;
���߼��: ��������У�˶ʿ��ҩʦ �о�����ҽԺҩѧ���ٴ�ҩѧ��ҩ�����ѧ ��ͨѶ��������֥��Ů����ʿ��˶ʿ����ʦ �о�����ҩ���¼������¼����о� Tel��0371��67781908 E-mail��wangyz@zzu.edu.cn
���ñ���:   
�����, ֣����, �����µ� .��«��B����֬��������������ĸϸ����SK-N-SHϸ��������ϸ������������[J]  �й�ҩѧ��־, 2012,V47(23): 1894-1898
XU Bing-Xin, ZHENG Jia-Xin, ZHAO Yuan-Yuan etc .Cytotoxicity Evaluation of Cucurbitacin B Solid Lipid Nanoparticles (SLN) on Human Neuroblastoma SK-N-SH Cell Line in Vitro[J]  Chinese Pharmaceutical Journal, 2012,V47(23): 1894-1898
��
[1] LI C Y��HOU S X��YANG K��et al. Study on quantitative determination of cucurbitacins B in plasma by HPLC [J]. Chin Pharm J (�й�ҩѧ��־)��2001��36��8����557-559.
[2] LZBICKI T�� MAZUR J��IZBICKA E. Epidemiology and etiology of neuroblastoma��An overview[J]��Anticancer Res�� 2003�� 23(1B)��755-760��
[3] MULLER R H�� RANJITA S�� KECK C M. 20 Years of lipid nanoparticles (SLN & NLC)�� Present state of development & industrial applications[J]. Current Drug Discovery Technologies��2011��8(3)�� 207-227.
[4] WANG Y Z�� DENG Y H�� MAO S R�� et al. Characterization and body distribution of ��-elemene solid lipid nanoparticles (SLN)[J]. Drug Dev Ind Pharm��2005��31(8)��769-778.
[5] WANG J X��SUN X�� ZHANG Z R. Enhanced brain targeting by synthesis of 3�䣬5��-dioctanoyl-5-fluoro-2��-deoxyuridine and incorporation into solid lipid nanoparticles[J]. Eur J Pharm Biopharm�� 2002�� 54(3)�� 285-290.
[6] WANG Y Z��ZHOU J��ZHENG J X��et al. Preparation and tissue distribution of folate receptor-targeted solid lipid nanoparticle for ��-elemene in rats[J]. Chin Pharm J(�й�ҩѧ��־)��2009��44(12)��920-925.
[7] SHI Y J��ZENG K��LI G F��et al. Cellular uptake and apoptosis induction of podophyllotoxin-loaded solid lipid nanoparticles in human keratinocyte [J]. Shandong Med J (ɽ��ҽҩ)��2010��50��48����25-26.
[8] MIAO J��ZHANG X G��JIANG S P��et al. Antiviral effect of adefovir dipivoxil loaded solid lipid nanoparticles in vitro [J]. Chin Pharm J(�й�ҩѧ��־)��2009��44��11����853-856.
[9] HU Z H�� ZHOU Y�� ZHANG X N. Investigation on anticancer activity of nanoparticles loaded lactosyl-norcantharidin [J]. Chin Tradit Her Drugs(�в�ҩ)��2010��41(12)��2005-2010.
[10] VOLAND C��BORD A��PELERAUX A��el al��Repression of cell cycle��related proteins by oxaliplatin but not cisplatin in human colon cancer cells[J]��Mol Cancer Ther��2006��5(9)��2149-2157��
[11] NURSE P.Cyclin dependent kinases and cell cycle control(noble lecture) [J]��Chem Bio Chem�� 2002��3��7����596-603��
[12] NISHITANI H��LYGEROU Z��Control of DNA replication licensing in a cell cycle[J]��Genes Cells��2002,7(6)��523-534��
[13] ZHANG M X��ZHANG H L��SUN C Y��et al. In vitro and in vivo inhibitory effect of cucurbitacin B on the growth of breast cancer cells [J]. Modern Oncology���ִ�����ҽѧ����2009,17(1)��16-19.
[14] LIU T Y�� ZHANG M X�� DENG Y H�� et al. Effects of cucurbitacin B on cell proliferation and apoptosis in Hep-2 cells [J]. J Clin Otorhinolaryngol Head Neck Surg���ٴ������ʺ�ͷ�������־����2008��22��9 ����403-407.
[15] TANNIN-SPITZ T�� GROSSMAN S�� DOVRAT S�� et al. Growth inhibitory activity of cucurbitacin glucosides isolated from citrullus colocynthis on human breast cancer cells[J]. Biochem Pharmacol�� 2007��73(1) ��56-67.
[1] ��ӷ棬�����棬����ƽ.N-�л�-3��5-�������Ǽ׻���-4-���ͪ������ĺϳɼ���ϸ�����Գ����о�[J]. �й�ҩѧ��־, 2012,47(19): 1588-1591
[2] ����, ����, ����, κ��, ̷����, Ҧ��, ���ɯ.Ҷ�����������ͪ֬������Ʊ����������ۺ�ҩ��ѧ�о�[J]. �й�ҩѧ��־, 2012,47(12): 979-983
[3] ������ л�� �찮�� ������ ������ ��һ.�ض�������ӻ���׶����������������Ʊ����������о�[J]. �й�ҩѧ��־, 2011,46(18): 1427-1431
[4] ���ջ� ��ӱ�� ����� �δ���.Ҷ�������ɼ���ۺ����������ݵ��Ʊ����俹���������о�[J]. �й�ҩѧ��־, 2010,45(22): 1742-1748
[5] ���ε� �·�Ӣ �ݺ���.���������ӻ��򴫵��������ϩ�ǰ�-��������ϸ��תȾ�о�[J]. �й�ҩѧ��־, 2010,45(18): 1368-1371
[6] ����;����;����;��չ��;��ѧũ .ȥ�װ�����N-�������Ǿ������������Ʊ������⿹��������[J]. �й�ҩѧ��־, 2009,44(12): 913-919
[7] ��ˮ��;���׷�;������;������;���ƻ�.�����������������Bͻ���嵰��D9N�ı�����������ѧ���Ե��о�[J]. �й�ҩѧ��־, 2009,44(11): 810-814
[8] ���˹�;�����.Pluronic P105/PEG-PE/TPGS��Ͻ���ϵͳ��ϲ���������[J]. �й�ҩѧ��־, 2009,44(10): 762-765
[9] ��ˮ��;ë�Ƿ�;������;�Ͻ�.�����������������B���ױ�������������Ե�ʵ���о�[J]. �й�ҩѧ��־, 2008,43(08): 568-572
[10] ˧��ƽ;���ҹ�;�½���;�β���;����Ȩ;�߽���.�Ǿ�������֬������Ʊ��������о�[J]. �й�ҩѧ��־, 2007,42(15): 1159-1163
[11] ������;�½���;�η�;����.˿��ù��C�������ϩ��������������ϸ�����Ե��о�[J]. �й�ҩѧ��־, 2004,40(10): 758-759
[12] ��绪 ��ѩ�� ��ȫʤ ���.�Ͼ���-�������ż������Ʊ��������о���[J]. �й�ҩѧ��־, 2000,35(07): 455-457
[13] ��־ï;ӹһ;����ƽ����;�ɱ���;���Тһ;�鴨����.ţϥ�߷������ʵ�ϸ�����Լ�����ɵij����о�[J]. �й�ҩѧ��־, 1999,34(05): 299-301
Copyright 2010 by �й�ҩѧ��־