Abstract��
OBJECTIVE To investigate the effect of spearmint oil on airway inflammation and the expression of interleukin-8��IL-8�� and CC-chemokines receptors 2��CCR2�� in rats with chronic obstructive pulmonary disease(COPD). METHODS COPD model was induced by intratracheal instillation of lipopolysaccharide(LPS) and passive smoking for 12 weeks in rats��and COPD rats were treated with spearmint oil for 4 weeks��After COPD was induced��the methacholine (Mch)-induced airway resistance (RL) and total and differential cell count in bronchoalveolar lavage fluid(BALF) were measured .The pathologic changes of lung tissue were observed by HE��AB-PAS and Masson staining ��and IL-8 content and CCR2 expression were measured. The effect of spearmint oil was determined. RESULTS Spearmint oil 30��100 and 300 mg��kg-1 significantly reduced leucocyte�� neutrophile �� lymphocyte and macrophage numbers in BALF��and attenuated bronchiolitis��pulmonary interstitial inflammation and inflammation cell infiltration��and decreased the destruction of pulmonary alveolus and the thickness of bronchioles walls��and inhibited goblet cell proliferation. Spearmint oil 100 and 300 mg��kg-1 significantly reduced IL-8 in lung homogenate and spearmint oil 30��100 and 300 mg��kg-1 significantly decreased the expression of CCR2 in lung tissues��CONCLUSION Spearmint oil has protective effect on lung injury in COPD rats��since it improves pulmonary inflammation��goblet cell proliferation �� collagen deposition��emphysema��airway remodeling. The mechanism may include reducing IL-8 content and decreasing CCR2 expression in lung tissue.
.Effect of Spearmint Oil on Airway Inflammation�� Interleukin-8 and CCR2 Expression in Lung Tissue of COPD Rats[J] Chinese Pharmaceutical Journal, 2010,V45(6): 423-427
2010, Vol. 45 