Abstract��
OBJECTIVE To develop a specific and accurate reversed-phase HPLC-UV method for simultaneous determination of fenofibrate (FEF and its metabolite fenofibric acid (FEFA in rats plasma and lymph fluid�� then the method was applied in the study of intestinal lymphatic transport of fenofibate in rats. METHODS The intestinal mesenteric lymph duct and jugular vein of unconscious rats was cannulated for collecting blood and lymph fluid after intraduodenal adminstration of a single dose of 30 mg��kg-1 fenofibrate suspension. Plasma and lymph fluid samples were pretreated by protein precipitant containing internal standard naproxen and then analysed by the validated HPLC method. The analytical column was a Calesil ODS (4.6 mm��150 mm�� 5 ��m. The mobile phase was composed of methanol and 0.4�� phosphoric acid solution (pH 3.2 (82��18. The flow rate was 1.0 mL��min-1. The wavelength of UV-detector was set at 286 nm. RESULTS The retention time of FEFA�� FEF and IS were 4.25��9.15 and 3.3 min�� respectively. The calibration curves showed good linear regression in the range of 0.2-50 and 0.1-25 ��g��mL-1(r>0.999 for plasma and lymph fluid samples. Intra-and inter-day variations were less than 9���� accuracy was 95��-110���� and extraction recovery was more than 84��. Both FEFA and FEF were detected in lymph fluid samples�� while in plasma only FEFA was found.The values of ��maxin plasma and lymph fluid samples were (13.29��2.17�� (4.35��0.97�� and (0.51��0.13 ��g��mL-1�� respectively and tmax were (5.67��2.08�� (4.75��0.96�� and (3.75��1.26 h�� respectively. CONCLUSION The established and validated RP-HPLC-UV method is simple��sensitive and accurate for simultaneous determination FEF and FEFA in rats plasma and lymph fluid. The absorbed fenofibrate can be partially transported through mesenteric lymphatic routes into systemic circulation in rats.
.Determination of Fenofibrate and Its Metabolite by HPLC and Its Application in Rat Intestinal Lymphatic Transport[J] Chinese Pharmaceutical Journal, 2011,V46(9): 686-690
2011, Vol. 46 