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�й�ҩѧ��־ 2011, Vol. 46 Issue (21) :1674-1676    DOI:
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HPLC�ⶨ�����ж������༰��������
������1,2�����춫2��л��1,2������ƽ1,2��÷�˹�2*
1. �人���̴�ѧ��������ҩѧԺ���人 430073��2. ����ҽѧ��ѧԺ����ҩ���о��������� 100850
WANG Zeng-ming1,2��GUO Qing-dong2��XIE Fang1,2��WANG Xing-ping1,2��MEI Xing-guo2*
1. School of Chemical Engineering and Pharmacy�� Wuhan Institute of Technology�� Wuhan 430073�� China�� 2. Institute of Pharmacology and Toxicology�� Academy of Military Medical Sciences�� Beijing 100850�� China

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ժҪ Ŀ�� ������ЧҺ��ɫ�׷��ⶨ������������Ҫ��������ɷֱ�ûʳ�Ӷ�����ûʳ��������EGCG�����������ûʳ��������ECG���������ɷֿ�����CAF������������������ ɫ����ΪAgilent ZORBAX SB-C18����4.6 mm��250 mm��5 ��m���������ࣺAΪ���棬BΪ0.05%����ˮ��Һ���ݶ�ϴ��������0~18 min��12%A��18~20 min��12%A��16%A��20~35 min��16%A�����٣�1 mL��min-1�����£�30 �棻��Ⲩ����278 nm����� ������EGCG��ECG��CAF�����Է�Χ�����ϵ���ֱ�Ϊ159.4��797.0 mg��L-1 (r=0.999 6)��40.16��120.18 mg��L-1 (r=0.999 7)��1.6��12.8 mg��L-1 (r=0.999 9)�������޷ֱ�Ϊ0.8��0.8��1.0 ng������޷ֱ�Ϊ0.25��0.25��0.30 ng��ƽ�����������ʷֱ�Ϊ98.94% ��RSD=0.72%����99.45% ��RSD=1.00%����100.46%��RSD=0.83%�����������ܶȼ��ظ������ã�RSD��С��2%������ ������������㡢����׼ȷ�������Ժã������ڲ�����EGCG��ECG��CAF�����IJⶨ��
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Abstract�� OBJECTIVE To establish a HPLC method for the simultaneous determination of two main catechins (EGCG and ECG) and caffeine (CAF) which is a content limited substance in tea polyphenols. METHODS The separation was performed on a C18 column (Agilent ZORBAX SB-C18��4.6 mm��250 mm��5 ��m) with the mobile phase consisting of acetonitrile��A��-0.05% phosphoric acid aqueous solution��B��by gradient elution. The gradient condition was as follows�� 0-18 min��A��12%��18-20 min��A��12%-16%��20-35 min��A��16%. The flow rate was 1 mL��min-1. The column temperature was set at 30 �棬and the UV detection wavelength was 278 nm. RESULTS The linear ranges and correlation coefficients of EGCG��ECG and CAF in tea polyphenols were 159.4-797.0 mg��L-1 (r=0.999 6)��40.16-120.18 mg��L-1 (r=0.999 7) and 1.6-12.8 mg��L-1(r=0.999 9) respectively. The limits of quantitation were 0.8��0.8 and 1.0 ng respectively. The limits of detection were 0.25��0.25 and 0.30 ng respectively. The average recoveries were 98.94%��99.45% and 100.46% with corresponding RSDs of 0.72%��1.00% and 0.83%��respectively. The RSDs were smaller than 2.0%. CONCLUSION The method is simple��accurate��reproducibility and can be used to determine EGCG��ECG and CAF in tea polyphenols.
Keywords�� HPLC,   tea polyphenols,   catechin,   caffeine,   content determination     
�ո�����: 2011-11-11;
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������1, 2, ���춫2�� .HPLC�ⶨ�����ж������༰��������[J]  �й�ҩѧ��־, 2011,V46(21): 1674-1676
WANG Zeng-ming, GUO Qing-dong, XIE Fang etc .Determination of Catechins and Caffeine in Tea Polyphenols by HPLC[J]  Chinese Pharmaceutical Journal, 2011,V46(21): 1674-1676
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