目的：测定兔血浆及微量淋巴組织中阿霉素的含量，揭示阿霉素脂质体的靶向定位及生物体内的药物代谢 动力学特性。方法：取样量，血浆为0.5ml，淋巴組织为0.1g，加入磷酸盐緩冲液1ml(0.2mol/L，pH7.8)于冰浴 中超声匀浆，以盐酸柔红霉素为内标，采用有机相[氯仿-甲醇（4 ：1)]一步提取，40℃下氮气吹干，残渣用甲醇溶解， 进样75μl色谱条件为YWG-C18柱（150mmΧΦ4.6mm，粒径为10μm);流动相为甲醇-L腈-0.01mol/L磷酸二氢铵-冰醋酸（50：22：28：0.5);流速为1.2ml/min，激发波长为479nm，发射波长为587nm,检測器灵敏度为 H;室温下操作。结果：本方法线性范围：血浆阿霉素为10-1000ng/ml;淋巴组织阿霉素为0.05-5.0μg/g，两者 的线性相关系数r=0.9999(n=6);日内及日间重现性：RSD%在2.10-6.15之间；药物自血浆及淋巴组织的平均 回收率分别为100.11%和100.5%;灵敏度：血浆及淋巴组织中最低检測浓度分别为2ng/ml和0.025μg/g;方法 简便快速，一个样本从提取处理到色谱分离出结果，可在30min内完成。结论：本方法能够簡便、准确地測出兔血浆 及微量淋巴组织中阿霉素的含量，适于淋巴给药后阿霉素生物体内药物代谢动力学的研究。

OBJECTIVE ： To determine the concentrations of adriamycin in rabbit plasma and lymph nodes. METHOD ： After addition of the internal standard (IS； daunorubicin), a single-step extraction was performed with chloroform-methyl alcohol (4:1). The organic layer was evaporated and the remains was redissolved with 100 methyl alcohol. The drug and IS were chromatographed on C18 column with a mobile phase consisting, of methyl alcohol ：acetonitrile ：0.01mol/L ammonium dihydric phosphate ：acetic acid(50:22:28: 0.5) at a flow rate of 1.2 ml/min and detected by fluorescence detector at excitation and emission wavelengths of 479 and 587 nm,(respectively. RESULTS ： A better linearity was obtained from 10 to 1 000 ng of adriamycin per ml for plasma and 0.05 to 5.0 μg/g for lymph nodes with a good correlation coefficient(r = 0. 999 9,n = 6). The intra and interday coefficients of variation were always<6.15%. The mean recovery of adriamycin from plasma and lymph nodes was 100. 11 % and 100.5% ,respectively. The improved method was very sensitive; the minimum detectable concentration was 2 ng/ml for plasma and 0. 025 μg/g for lymph nodes, respectively. CONCLUSION ： This method is suitable for pharmacokinetics studies in rabbits.