Abstract��
OBJECTIVE:To analyze the distribution of weight��average molecular weight (r) of four ��Angelica polysaccharides isolated from Angelica sinensis (Oliv.)Diels:AP����AP-��AP-��AP�� and determine their characters of electric��charge,in order to provide a practical analytical method for studying the constituent��activity relationship of polysaccharides. METHODS:Two kinds of high performance liquid chromatography methods were employed,including high��performance gel filtration chromatography for r determination and high performance anion exchange chromatography for electric charge analysis.Improved phenol sulfuric acid method combined with ELISA reader were used for the polysaccharide fractions determination,and Sever blue G dye��combined method for protein fractions as well. RESULTS:Each of the four Angelica polysaccharides,AP-��AP-��AP-�� and AP-��,was composed of 4��5 polysaccharide constituents with different r distribution and characters of electric charge;and there were small amount of different free or combined proteins in the four Angelica polysaccharides. CONCLUSION:The four Angelica polysaccharides,AP-��AP-��AP-�� and AP-��,are different from each other in r and character of electric��charge;this method is practical and reliable for the study of constituent activity relationship of polysaccharides.
2000, Vol. 35 
