Abstract��
OBJECTIVE To establish a HPLC method for the simultaneous determination of two component concentrations of complex chlorzoxazone tablets in human serum. METHODS The serum samples were precipitated with saturated Zinec sulfate solution,extracted with Ether,dried with Nitrogen gas,dissolved in Methanol and determined with HPLC.The Hypersil ODS C18column was used.The mobile phase consisted of methanol water (45��55) phenacetinum was used as the internal standard.It was detected at 280 nm. RESULTS A linearity was obtained from 1.0 to 32.0 ��g��ml-1of chlorzoxazone (r=0.9994) and from 1.2 to 38.4 ��g��ml-1of acetaminophen (r=0.9999),respectively.The limits of detection were 0.5 and 0.6 ��g��ml-1,respectively.Their average recoveries were 95.3% and 91.6%,respectively.The precision (RSD) were 6.0% and 5.1%,respectively. CONCLUSION This study provided a simple and reliable assay which can be used to simultaneously determine two component concentrations of complex chlorzoxazone tablets in human serum and to reseach their pharmacokinetics and bioavailability.
.Determination of two component concentrations of complex chlorzoxazone tablets in human serum by HPLC[J] Chinese Pharmaceutical Journal, 2000,V35(11): 763-765
2000, Vol. 35 
