Abstract��
OBJECTIVE To observe the inhibition effect of safflor yellow on the platelet aggregation,5-HT relation,intercellular free calcium concentration elevation induced by platelet activating factor (PAF). METHODS Safflor yellow was prepared from water abstract of Carthamus tinctorius L.with silicon gel adsorption.The platelet aggregation and 5-HT relation were studied by turbidimetry and OPT fluorescence assay.The inner free calcium concentration was measured with fura-2/AM probe fluorescent technique. RESULTS It was shown that the inhibition effects of safflor yellow on PAF (2.0��10-9mol��L-1)induced platelet aggreation and platelet 5-HT release were dose-dependent.The inhibitive incidences of safflor yellow (0.21,0.42,0.85,1.69 g��L-1) on platelet aggregation or 5-HT release were 28.0%,32.9%,��60.0%��,79.4% or 4.74%,12.9%,32.9%,58.4%,respectively.It was found that safflor yellow (4.1,6.5,10.3,16.7,26.7 mg��L-1) inhibited the elevation of inner free calcium concentration of platelet evoked by PAF (8.0��10-10 mol��L-1) dose-dependentently. CONCLUSION The rabbit platelet activation induced by platelet activating factor can be inhibited by safflor yellow.
.Inhibition of safflor yellow on rabbit platelet activation induced by platelet activating factor[J] Chinese Pharmaceutical Journal, 2000,V35(11): 741-744
2000, Vol. 35 
