Abstract��
OBJECTIVE To study the isolation and composition of Aconitum kusnezoffii�� Reichb.polysaccharkide(AK). METHODS AK was purified by DEAE ion-exchange cellulose(DEAE-52) and sepharose Cl-4B.The component of the saccharide was determined by GC and TLC.Its MW was measured by gel filtration.The form of glucosidic bond was detrermined by IR.The content of the saccharide was measured by colorimetry. RESULTS Rhamnose,xylose,mannose,glucose,galactose and arabinose were included in AK with the molar ratio of 1��1.7��203��1.7��59.7��3.5.Its average MW was estimated t be 2.8��104.The content of saccharide was 88%.Typical absorption of polysaccharide was shown in its IR spectrum.It contained ��-glucosidic bonds by IR analysis.It had no typical absorption of nucleic acid or protein by UV scanning. CONCLUSION AK is first isolated from the root of Aconitum kusnezoffii Reichb..
2000, Vol. 35 
