Abstract��
OBJECTIVE To induce the hairy roots autotetraploid Isatis indigotoca Fort.and discuss the probability to produce useful botanic secondary metabolites from hairy root culture on a large scale. METHODS The cotyledon leaf explants of autotetraploid Isatis indigotoca Fort.were infected by Agrobacterium rhizogenes strain A4,and five antiendotoxic active organic acids ��Anthranilic acid,salicylic acid,syringic acid,benzoic acid and 3-(2-carboxyphenyl)-4(3H)-quinazolinone�� were determined simultaneously by high performance capillary electrophoresis. RESULTS The hairy roots of autotetraploid Isatis indigotica Fort.could be indced by Agrobacterium rhizogenes strain A4 and the hairy roots contained same five antiendotoxic active components as that in the primary plant. CONCLUSION Agrobacterium rhizogenes could induce hairy roots from polyploid plants and the hairy roots might be utilized to produce valuable botanic secondary metabolites.
.Studies on culture of hairy roots of autotetraploid Isatis indigotoca and analysis of its antiendotoxic active components[J] Chinese Pharmaceutical Journal, 2000,V35(11): 728-731
2000, Vol. 35 
