目的探讨雷公藤甲素(triptolide,TL)对培养的大鼠气道平滑肌细胞(ASMC)增生及原癌基因c-fos与c-jun表达的影响。方法按不同药物分为6组，TL组(浓度分别为5，20，80，320，1 280 μg·L-1)及地塞米松1 mg·L-1组，采用MTT法检测不同浓度药物对ASMC增殖的影响，同时进行逆转录-聚合酶链反应(RT-PCR)检测各组c-fos，c-jun 的 mRNA表达水平。结果MTT方法显示TL对ASMC增殖有明显抑制作用，并呈剂量依赖性，TL的IC50为51.52 mg·L-1；RT-PCR方法显示TL浓度分别为80，320，1 280 μg·L-1和地塞米松1 mg·L-1能显著抑制c-fos，c-jun mRNA表达。结论TL有抑制ASMC增生的作用，其机制可能与下调c-fos和c-jun表达有关。
Abstract
OBJECTIVE To investigate the effects of triptolide on airway smooth muscle cells (ASMC) proliferation and expressions of oncogene c-fos and c-jun in culturing ASMC.METHOD S The primary ASMC from rat were collected and cultured and studied.The effects of triptolide at different doses (5,20,80,320 and 1 280 μg·L-1) and dexamethasone (1 mg·L-1) on ASMC proliferation were studied by MTT method.The mRNA expressions of c-fos and c-jun form ASMC were examined by RT-PCR.RESULTS The ASMC proliferation was inhibited at the dose (IC50=51.52 mg·L-1).The mRNA expression of c-fos and c-jun in ASMC was also inhibited by triptolide (80,320 and 1 280 μg·L-1) and dexamethasone.CONCLUSION S It suggested that triptolide remarkably inhibited ASMC proliferation probably by suppressing the expression of c-fos and c-jun.
关键词
雷公藤甲素 /
原癌基因 /
支气管哮喘
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Key words
triptolide /
oncogene /
asthma
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参考文献
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脚注
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基金
广东省高教厅课题（9735）
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