Abstract��
OBJECTIVE To establish a RP-HPLC method for the simultaneous determination of four isoquinoline alkaloids in the root of Dactylicapnos scandens. METHODS The samples was separated on a Hypersil ODS C18 column (4.6 mm��250 mm, 5 ��m) with gradient elution. The mobile phase was composed of methanol and 0.2% phosphoric acid(adjust to pH 7.0 with triethylamine). The flow rate was 1.0 mL��min-1 and the UV absorbance detection was set at 265 nm. RESULTS Four alkaloids were separated well. Good linearies were obtained within the range of 0.007 3-0.065 7,0.172 2-4.304,0.486-4.86 ��g and 0.4870-9.739 2 ��g for sinomenine, protopine, corydine and isocorydine respectively. The average recoveries were 97.84% (RSD=0.89%), 98.98%(RSD=0.58%), 97.14% (RSD=1.13%) and 97.65%(RSD=0.74%). CONCLUSION The method is accurate, reliable and convenient. It can be used to control the quality of Dactylicapnos scandens and its products.
.Simultaneous Determination of Four Isoquinoline Alkaloids in Root of Dactylicapnos scandens by RP-HPLC [J] Chinese Pharmaceutical Journal, 2009,V44(03): 233-236
2009, Vol. 44 
