1.College of Life Sciences��China Jiliang University��Hangzhou 310018��China�� 2.College of Life and Environmental Sciences��Hangzhou Normal University��Hangzhou 310036 ��China
Abstract��
OBJECTIVE To establish an identification and quantification method in fermentation by GC.METHODS The trichodermin was determined by GC with HP-5 capillary column(5% phenyl methyl siloxane,30 m��320 ��m,0.25 ��m) with FID detector and nitrogen as the carries gas.The injector temperature was 250 ��,the detector temperature was 270 ��,the column temperature was increased by program,the initial temperature was 100 ��,maintained for 5 min,raised to 260 �� with a rate of 10 �桤min-1,maintained for 5 min.The flow rate of N2 was 1.5 mL��min-1.RESULTS Trichodermin was completely separated by GC,the linearity was in the range of(0.01��0.5) g��L-1,average recovery was 99.76%,RSD was 1.69%.CONCLUSION The method of GC was accurate,simple,rapid and producible.
2008, Vol. 43 
