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�й�ҩѧ��־ 2008, Vol. 43 Issue (15) :1130-1135    DOI:
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Fasͨ·���ȱ���������UVB�յ���HaCaTϸ�������е�����
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1.�ൺ��ѧҽѧԺ ɽ���ൺ 266071;2.�ൺ��ѧʦ��ѧԺ ɽ���ൺ 266071;3.�ൺ��ѧҽѧԺ����ҽԺ ɽ���ൺ 266002;4.�й�ˮ����ѧ�о�Ժ�ƺ�ˮ���о��� ɽ���ൺ 266071
LI Bing-hua1��WANG Hong-bing2��QUAN Xiang-hua3��WANG Yue-jun4��WANG Chun-bo1
1. Medical College��Qingdao University��Qingdao 266071��China�� 2.Normal College��Qingdao University��Qingdao 266071��China�� 3. Medical School Hospital��Qingdao University��Qingdao 266002��China��4. Yellow Sea Fisheries Research Institute��Chinese Academy of Fishery Sciences��Qingdao 266071��China

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ժҪ Ŀ�Ĵӵ�����ط���Fas(CD95)��Fas��������ṹ��(Fas associated protein with death domain,FADD)�������춬ø-8(caspase-8)�ĽǶ�,�о��ȱ�����(polypeptide from Chlamys farreri,PCF)����������B(Ultraviolet B,UVB)�յ����˽����γ�ϸ����(immortalized human keratino,HaCaT)ϸ�����������û��ơ�����ʵ�����Ϊ6��:�����顢UVBģ���顢UVB+5.68mmol��L-1ά����C���Զ����顢UVB+5.69mmol��L-1PCF�顢UVB+2.84mmol��L-1PCF�顢UVB+1.42mmol��L-1PCF�顣������ʵ�����ȷ��UVB�յ���HaCaTϸ������ģ��;��֬��������Ӿ��ӫ��Ⱦɫ(Ho-echst33258)����PCF��UVB�յ���HaCaTϸ��������Ӱ��;��֬��������Ӿ����caspase-8���Ƽ�(z-IETD-fmk)��UVB�յ���HaCaTϸ��������Ӱ��;��ת¼-�ۺ�ø����Ӧ(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)���Fas(CD95)mRNA�ı��;������ӡ�������FADD��caspase-8���׵ı����PCF����������UVB�����HaCaTϸ������;z-IETD-fmk��UVB�յ���HaCaTϸ��������������������;1.42��5.69mmol��L-1�ڵ�PCF�ɼ�������������UVB�����Fas,FADD�ı�����Ӽ�caspase-8�Ļ������PCF�ɼ�������������UVB�յ���HaCaTϸ������,�����û���������Fas,FADD�ı�Pcaspase-8�Ļ�йء�
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�ؼ����� �ȱ�����   Fas   Fas��������ṹ��   �����춬ø-8   HaCaTϸ��   ����     
Abstract�� OBJECTIVE To investigate the inhibition effect of polypeptide from Chlamys farreri(PCF)on from UVB-induced apoptosis of HaCat cells through Fas and Fas-associated protein with death domain(FADD)and caspase-8.METHODS Experiments design were divided into six groups:control group,UVB model group,UVB+5.68 mmol��L-1 vitamine C positive control group,UVB+5.69 mmol��L-1 PCF group,UVB+2.84 mmol��L-1 PCF group,UVB+1.42 mmol��L-1 PCF group.UVB-induced apoptotic model of HaCaT cells was established by orthogonal design.Using agarose gel electrophoresis and fluorescence staining(Hoechst 33258),the effects of PCF on UVB-induced apoptosis of HaCaT cells were investigated.Using agarose gel electrophoresis,the effects of caspase-8 inhibitor z-IETD-fmk on UVB-induced apoptosis of HaCaT cells were investigated.Expression levels of Fas(CD95)mRNA were detected by RT-PCR.Expression levels of FADD and caspase-8 were determined by Western blot analysis.RESULTS PCF significantly protected against UVB-induced apoptosis of HaCaT cells.z-IETD-fmk had inhibitory effects on UVB-induced apoptosis of HaCaT cells.PCF inhibited the expression of Fas and FADD and activation of caspase-8 dose-dependently.CONCLUSION PCF could protect HaCaT cells from UVB-induced apoptosis dose-dependently.Its inhibitory effect on apoptosis may attributes to inhibition expression of Fas and FADD and activation of caspase-8.
Keywords�� polypeptide from Chlamys farreri(PCF),   Fas,   FADD,   caspase-8,   HaCaT cell,   apoptosis      
�ո�����: 2007-08-08;
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�����, �����, ȫ�㻨�� .Fasͨ·���ȱ���������UVB�յ���HaCaTϸ�������е�����[J]  �й�ҩѧ��־, 2008,V43(15): 1130-1135
LI Bing-hua, WANG Hong-bing, QUAN Xiang-hua etc .Role of Fas Pathway in Polypeptide from Chlamys farreri Inhibiting UVB-Induced Apoptosis of HaCaT Cells [J]  Chinese Pharmaceutical Journal, 2008,V43(15): 1130-1135
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