SONG Rui-xia1��2��YU Jing1*��YANG Li-li1��CHANG Peng1��GUO Xue-ya1��LI Xiu-li1��WANG Han-qing3��DONG Chen-ming2
1.Department of Cardiology��Second Hospital of Lanzhou University��Lanzhou 730030��China��2. Department of Critical Care Medicine��Second Hospital of Lanzhou University��Lanzhou 730000��China�� 3. Center for Natural Medicine Developing of Gansu Province��Lanzhou Institute of Chemical Physics��Chinese Academy of Sciences��Lanzhou 730030��China
Abstract��
OBJECTIVE To investigate the effects of calycosin on the protein and gene expressions of angiotensin-converting enzyme(ACE) and angiotensin-converting enzyme 2(ACE2) in human umbilical vein endothelial cells(HUVECs).METHODS Cultured HUVECs were divided randomly into 5 groups:control,angiotensin��(Ang��)(1��10-6mol��L-1),Ang��(1��10-6mol��L-1)+calycosin(10,1,0.1 mg��L-1 respectively).Morphology of vascular endothelial cells was studied by common microscope with hematoxylin and eosin double staining.Changes in both protein and gene expression of ACE,ACE2 were detected with immunohistochemistry analysis and reverse transcription polymerase chain reaction(RT-PCR) technique respectively.RESULTS ��Compared with controls,Ang��(1��10-6mol��L-1) promoted both protein and gene expressions of ACE and inhibited the expression of ACE2 in HUVECs(P<0.05).��Calycosin induced a concentration dependent increase of both protein and gene expressions of ACE2(P<0.05),downregulated the expression of ACE(P<0.05).CONCLUSION Calycosin from Gansu Astragalus membranaceus showed a protective effect on HUVECs damage by inhibiting the product of ACE and increasing the expression of ACE2.The results suggest that calycosin may play an important role in prevention and treatment of vascular disease.
SONG Rui-xia,
YU Jing,
YANG Li-li etc
.Regulation Effect of Calycosin Extracted from Astragalus membranaceus of Gansu Province on the Expressions of ACE/ACE2 in Vascular Endothelial Cells [J] Chinese Pharmaceutical Journal, 2008,V43(08): 594-597
2008, Vol. 43 
