中国药学杂志
    
           首页  |  期刊介绍  |  编 委 会  |  投稿指南  |  期刊订阅  |  广告服务  |  会议信息  |  联系我们  | 
�й�ҩѧ��־ 2008, Vol. 43 Issue (08) :613-616    DOI:
���� ����Ŀ¼ | ����Ŀ¼ | ������� | �߼����� << | >>
RP-HPLC�ⶨBeagleȮѪ����2,3,5,4��-���ǻ�������ϩ-2-O-��-D-�������յ�Ũ�ȼ�ҩ��ѧ�о�
������;����;��ΰ;ʯ�α�;����ƽ;����ϼ
������ҽҩ��ѧ ���� 100102
WANG Fu-gang ��LIU Bin* ��WANG Wei ��SHI Ren-bing��CHEN Yu-ping ��WANG Yu-xia
Beijing University of Chinese Medicine��Beijing 100102��China

Download: PDF (0KB)   HTML (0KB)   Export: BibTeX or EndNote (RIS)      Supporting Info
ժҪ Ŀ�Ľ���BeagleȮѪ����2,3,5,4��-���ǻ�������ϩ-2-O-��-D-��������(SBGC)��RP-HPLC�����ⶨ����,�о�Bea-gleȮ��θ�����ڶ�����ϩ����Ч��λ������ҩ��ѧ������ɫ������:Agilent Zorbax Extend-C18ɫ����(4.6mm��250mm,5��m);������:����-ˮ(15:85);��Ⲩ��:320nm;����:30��;����:1.0mL��min-1��BeagleȮ��θ�����ڶ�����ϩ����Ч��λ��ʱȡѪ,�ⶨѪ��ҩ��Ũ��,����DAS�������ҩ��ѧ���������SBGC��0.2064��8.256mg��L-1�����Թ�ϵ����(r=0.9994);BeagleȮ��θ�����ڶ�����ϩ����Ч��λ1.0,1.5,2.0g��kg-1����϶��ҿ���ʽģ��,t1/2���ֱ�Ϊ0.20,0.10��0.14h;t1/2���ֱ�Ϊ0.56,0.60��0.64h;V/F�ֱ�Ϊ0.08,0.15��0.08L��kg-1;CL/F�ֱ�Ϊ0.11,0.20��0.16L��h-1�����۸÷����١���㡢׼ȷ,������ҩ��ѧ�о���Ҫ��
Service
�ѱ����Ƽ�������
�����ҵ����
�������ù�����
Email Alert
RSS
�����������
������
����
��ΰ
ʯ�α�
����ƽ
����ϼ
������
����
��ΰ
ʯ�α�
����ƽ
����ϼ
�ؼ����� ������   �����ЧҺ��ɫ�׷�   2   3   5   4��-���ǻ�������ϩ-2-O-��-D-��������   ������ϩ��   ҩ��ѧ     
Abstract�� OBJECTIVE To establish a RP-HPLC method for the determination of 2,3,5,4'-tetrahydroxystilbene-2-O-��-D-glucoside in Beagle dog plasma and to investigate the pharmacokinetics of the effective fraction of stilbene glycoside of Polygonum multiflorum after intragastric administration.METHODS The analysis was performed on a Agilent Zorbax Extend C18 column(4.6 mm��250 mm,5 ��m) at 30 �� with the mobile phase of acetonitrile-water(15��85) at a flow rate of 1.0 mL��min-1.The detective wavelength was 320 nm.The plasma samples were collected after intragastric administration of the effective fraction of stilbene glycoside of Polygonum multiflorum in Beagle dogs.The concentrations of 2,3,5,4'-tetrahydroxystilbene-2-O-��-D-glucoside in plasma were measured by RP-HPLC method and the pharmacokinetic parameters were calculated by DAS.RESULTS The calibration curve of 2,3,5,4'-tetrahydroxystilbene-2-O-��-D-glucoside was linear in the range of 0.206 4��8.256 mg��L-1 with the correlation coefficient of 0.999 4.The concentration-time curves of 2,3,5,4'-tetrahydroxystilbene-2-O-��-D-glucoside in plasma were fitted with a two-compartment open pharmacokinetic model after intragastric administration of the effective fraction of stilbene glycoside of Polygonum multiflorum in Beagle dogs.The main pharmacokinetic parameters of 2,3,5,4'-tetrahydroxystilbene-2-O-��-D-glucoside after a single dose of 1.0,1.5,2.0 mg��kg-1 of the effective fraction stilbene glycoside of Polygonum multiflorum in Beagle dogs plasma were:t1/2�� 0.20,0.10 and 0.14 h;t1/2�� 0.56,0.60 and 0.64 h;V/F 0.08,0.15 and 0.08 L��kg-1;CL/F 0.11,0.20 and 0.16 L��h-1.CONCLUSION The method is sensitive,stable and easy to process.It is practical and suitable for the pharmacokinetic study of 2,3,5,4'-tetrahydroxystilbene-2-O-��-D-glucoside.
Keywords�� Polygonum multiflorum,   RP-HPLC,   2,   3,   5,   4'-tetrahydroxystilbene-2-O-��-D-glucoside,   stilbene glycoside     
�ո�����: 2007-06-01;
��������:��ʮһ�塱���ҿƼ�֧�żƻ�(2006BAI08B03-04);��������ѧ�����о��ص���Ŀ(105029);������Ȼ��ѧ�������(90409011,30672598);��������Ȼ��ѧ�������(7061002)
ͨѶ���� ����   
���ñ���:   
������, ����, ��ΰ�� .RP-HPLC�ⶨBeagleȮѪ����2,3,5,4��-���ǻ�������ϩ-2-O-��-D-�������յ�Ũ�ȼ�ҩ��ѧ�о�[J]  �й�ҩѧ��־, 2008,V43(08): 613-616
WANG Fu-gang, LIU Bin, WANG Wei etc .Determination of 2,3,5,4'-Tetrahydroxystilbene-2-O-��-D-glucoside in Beagle Dogs Plasma and Its Pharmacokinetics by RP-HPLC [J]  Chinese Pharmaceutical Journal, 2008,V43(08): 613-616
��
û�б��IJο�����
[1] ���Σ���ռ�֣�������������ο��������裬 �����º���������.������Դ���Penicillium sacculum�μ���л������о�[J]. �й�ҩѧ��־, 2012,47(8): 577-580
[2] �������ᡤФ���ᣬ��ѧ��.ûʳ�Ӷ�����ûʳ��������С��Lewis�ΰ���ֳ�Լ�A549��Calu-3�ΰ���ֳ����������[J]. �й�ҩѧ��־, 2012,47(8): 585-589
[3] ��������������������ã�л����������.��ҩ�նԸʲ��ἰ�ʲݴ����������ҩ��ѧ��Ӱ��[J]. �й�ҩѧ��־, 2012,47(8): 599-602
[4] �ᄃ��������Ǯ��������������̫����������.HZ08�й����ʵ�ɫ��-���׽ṹ����[J]. �й�ҩѧ��־, 2012,47(8): 603-607
[5] ����, �����壬 ��ƣ� Ф����.3-��������-1��2��4-�������-1��4-����������ĺϳɼ����⿹�������ԵIJⶨ[J]. �й�ҩѧ��־, 2012,47(8): 639-641
[6] ��÷ ������.����ͪ����ǰ��֬�����Ƽ���Caco-2ϸ��ת���о�[J]. �й�ҩѧ��־, 2012,47(7): 529-533
[7] ��С�� ��ӱ ��½�� κӱ�� ��ǿ ���.�������������������Ʊ�����������ҩ��ѧ�о�[J]. �й�ҩѧ��־, 2012,47(7): 524-528
[8] ��ͬǿ DILEKOV J. ������ ���� ��Т�� ʯ�忭 ������ ���� �԰��� ��ʿ��.���ζ���3��4����ֵĵ�����ɷ��������Ǻ����ⶨ[J]. �й�ҩѧ��־, 2012,47(7): 551-554
[9] ������ ������ Фɺ ������ ���Ӣ.�������÷�ͬʱ�ⶨ��Ѫ����4�ַǵ��̴���̴�����[J]. �й�ҩѧ��־, 2012,47(7): 560-
[10] ��Ң ̷־�� ��ҽ�� ������ ��ΰ �ܺ��.Һ��ɫ�״������׷��ⶨ����Ѫ��ά������Ũ�ȼ������Ч�������о�[J]. �й�ҩѧ��־, 2012,47(7): 546-550
[11] ������ ��÷ ����Ӣ.�ຣ��������ȺCYP2C19�����̬���о�[J]. �й�ҩѧ��־, 2012,47(7): 539-542
[12] ����� ���޽� ���㻪 ���� .߻���ͪ�������������л�����ڴ�������ҩ��ѧ��Ӱ��[J]. �й�ҩѧ��־, 2012,47(6): 458-461
[13] ������ ��־�� ��ΰ�� �޶� ��� ���� .��ЧҺ��ɫ�׼����ҩ������Ʒ����ӱ��ͱ��׵�8���򾲴���ҩ���о�[J]. �й�ҩѧ��־, 2012,47(6): 462-466
[14] ������ ��� ������ ������ �̱��� .��ЧҺ��ɫ�׷�ͬʱ�ⶨ�����䲻ͬ����Ʒ��5�ֽ����صĺ���[J]. �й�ҩѧ��־, 2012,47(6): 471-474
[15] ���� ���� ����� ��Ԫ�� ��·ƽ ������ .��é�з�����ɷָ�ЧҺ��ɫ�׷��ⶨ�����Ľ���[J]. �й�ҩѧ��־, 2012,47(5): 375-379
Copyright 2010 by �й�ҩѧ��־