New Polyketides from Chemical Mutant Strain of Sponge-Associated Fungus Emericella variecolor XSA-07-2
HAN Peng-jie1, LONG Hai-lin2, LIU Dong2*, WU Zi-wei1, SUN Kun-lai1*
1. School of Food and Medical, Zhejiang Ocean University, Zhoushan 316002, China; 2. State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China
Abstract：OBJECTIVE To study the new bioactive secondary metabolites of diethyl sulfate chemical mutant strain of sponge-associated fungus Emericella variecolor XSA-07-2. METHODS Diethyl sulfate was used to make chemical mutagenesis of strain XSA-07-2, and one mutant strain M8 was chosen for large-scale fermentation to generate new secondary metabolites. The compounds were isolated and purified by chromatography on silica gel and ODS reversed-phase column and semi-preparative HPLC techniques. And their structures were identified by their physicochemical properties and NMR, MS data analysis. RESULTS Three new polyketides 1-3 were isolated from the extract of the solid fermentation culture of mutant strain M8. Compound 3 showed moderated antioxidant activity with IC50 of (13.58±0.14) μg·mL-1 by DPPH assay. CONCLUSION Diethyl sulfate chemical mutagenesis can stimulate sponge-associated fungus Emericella variecolor XSA-07-2 mutant strain M8 to produce new antioxidant polyketides.
韩鹏杰, 龙海林, 刘东, 吴紫薇, 孙坤来. 海绵共生真菌Emericella variecolor XSA-07-2化学诱变突变株中的新颖聚酮类化合物[J]. 中国药学杂志, 2019, 54(15): 1221-1225.
HAN Peng-jie, LONG Hai-lin, LIU Dong, WU Zi-wei, SUN Kun-lai. New Polyketides from Chemical Mutant Strain of Sponge-Associated Fungus Emericella variecolor XSA-07-2. Chinese Pharmaceutical Journal, 2019, 54(15): 1221-1225.
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2019, Vol. 54 
