Determination of Methylene Blue in Pig Plasma Using RP-HPLC with Solid Phase Extraction
LI Naa, SHI Xiao-jina*, GUO Feng-huab, WANG Zhi-mingb, CUI Xue-yana, ZHONG Ming-kanga
Author information+
a.Clinical Pharmacy Laboratory of Department of Pharmacy；b. Department of General Surgergy， Huashan Hospital Affiliated to Fudan University，Shanghai 200040，China
OBJECTIVE To establish a HPLC method for the determination of methylene blue in plasma. METHOD The drug was extracted from plasma with C2 column by SPE method. Separation was performed on a Kromasil C18 reverse-phase analytical column (4.6 mm×250 mm, 5 μm maintained at 40 ℃. The mobile phase consisted of acetonitrile-0.3% triethylamine of aqueous solution with the pH adjusted to 2.07 by phosphoric acid (31∶69, at a flow rate of 1 mL·min-1. The UV detector used a variable wavelength that was set at 668 nm for the first 6.7 min to detect methylene blue and then switched to 254 nm until 10 min to detect phenacetin. Phenacetin was used as internal standard for the quantitation. RESULTS The good linearity of methylene blue was obtained in the range of 8.55-855 μg·L-1. The correlation coefficient was 0.999 5 and the lower limit of quantification was 8.55 μg·L-1.The extraction recoveries were in the range of 81.08%-93.57%. The precisions of intra-batch and inter-batch were 3.93%-6.75% and 10.16%-13.36%, respectively. CONCLUSION The method is simple, sensitive, accurate and provides a research basis for the development of new application of methylene blue in medicine.
LI N;SHI Xio-jin;GUO Feng-hu;WNG Zhi-ming;CUI Xue-yn;ZHONG Ming-kng.
Determination of Methylene Blue in Pig Plasma Using RP-HPLC with Solid Phase Extraction[J]. Chinese Pharmaceutical Journal, 2011, 46(3): 231-234
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] AKOACHERE M，BUCHHOLZ K，FISCHER E，et al. In vitro assessment of methylene blue on chloroquine-sensitive and-resistant Plasmodium falciparum strains reveals synergistic action with artemisinins[J]. Antimicrob Agents Chemother，2005，49(11：4592-4597. [2] TURNER A R，DUONG C D，GOOD D J. Methylene blue for the treatment and prophylaxis of ifosfamide-induced encephalopathy[J]. Clin Oncol，2003，15(7：435-439. [3] SEGHATCHIAN J，WALKER W H，REICHENBERG S. Updates on pathogen inactivation of plasma using theraflex methylene blue system[J]. Transfus Apher Sci，2008，38(3：271-280. [4] BLAZ-DAVID N，DECOSTERD L A， APPENZELLER M，et al. Spectrophotometric determination of methylene blue in biological fluids after ion-pair extraction and evidence of its adsorption on plastic polymers[J]. Eur J Pharm Sci，1997，5(6：335-345. [5] XIE R F，TANG W L，HUANG Y W. Determination of methylene blue in human plasma by solid phase extraction[J]. Chin J Biol（中国生物制品学杂志，2005，18(5：424-425. [6] MA Y，YANG X L， SHANG J C，et al. Determination of a trace level of methylene blue in human plasma by combination of solid-phase extraction and sepctrophotometry[J]. Chin J Pharm Anal（药物分析杂志，2002，22（5：380-383. [7] BORWITZKY H，HAWFELI W E，BURHENNE J. Analysis of methylene blue in human urine by capillary electrophoresis[J]. J Chromatogr B，2005，826(1-2：244-251. [8] PETER C，HONGWAN D，KPFER A，et al. Pharmacokinetics and organ distribution of intravenous and oral methylene blue[J]. Eur J Clin Pharmacol，2000，56(3：247-250. [9] GAUDETTE N F，LODGE J W. Determination of methylene blue and leucomethylene blue in male and female fischer 344 rat urine and B6C3F(1 mouse urine[J]. J Anal Toxicol，2005，29(1：28-33. [10] ZHOU M M， LI X T， GENG L J，et al. HPLC Determination of lidocaine and methylene blue in rabbit plasma[J]. Chin J Pharm Anal（药物分析杂志，2007，27（9：1374-1377. [11] WANG R Y ，ZHOU X P， DU S. Determination of methylene blue in blood by HPLC and SPE[J]. Chin J Health Lab Technol（中国卫生检验杂志，2008，18(10：2012-2013. [12] QIAO W B， SHEN H， CUI X. Determination of methylene blue in human plasma by SPE-HPLC[J]. Qilu Pharm Affairs（齐鲁药事，2008，27(10：660-662. [13] LI S Z，ZHANG B， YUE X Y. Determination of methylene blue in serum by high performance liquid chromatography[J]. J Anal Sci（分析科学学报，2006，22（1：119-120. [14] RENGELSHAUSEN J，BURHENNE J，FRHLICH M，et al. Pharmacokinetic interaction of chloroquine and methylene blue combination against malaria[J]. Eur J Clin Pharmacol，2004， 60(10：709-715. [15] BURHENNE J， RIEDEL K D， RENGELSHAUSEN J，et al. Quantification of cationic anti-malaria agent methylene blue in different human biological matrices using cation exchange chromatography coupled to tandem mass spectrometry[J]. J Chromatogr B，2008，863(2：273-282. [16] LU X B, REN B, XIAO L, et al. Determination of lamotrigine in human plasma by HPLC[J]. Chin Pharm J（中国药学杂志，2008，43（2：232-233.
PDF(628 KB)
