摘要
目的 研究不同处方的葫芦素B半乳糖化肝靶向脂质体的体外溶血性。方法 通过常规体外试管法并结合显微镜下的红细胞形态学观察对3个处方的葫芦素B半乳糖化肝靶向脂质体的溶血作用进行评价，并通过筛选合适的破乳剂，应用分光光度法于575 nm下测定其溶血度。结果 肉眼观察3个葫芦素B半乳糖化肝靶向脂质体处方均无明显溶血现象，以10%Triton X-100为破乳剂通过分光光度法测定各处方的溶血度均小于5%。结论 3个葫芦素B半乳糖化肝靶向脂质体处方的溶血性符合注射剂要求，常规体外试管法同显微观察及分光光度法联合使用，可更准确地对制剂的溶血性进行判断，10% Triton X-100可做为脂质体溶血实验的优良破乳剂。
Abstract
OBJECTIVE To investigate hemolysis caused by the interaction between rabbit erythrocytes and cucurbitacin B galactosylated liver-targeting liposomes prepared with different adjuvants. METHODS The hemolytic phenomena of the three cucurbitacin B galactosylated liver-targeting liposomes formulations were observed by macroscopic observation and the optical microscope photos of the rabbit erythrocytes were taken with digital microscope. After being treated with a proper emulsion-breaker， the hemolysis percentage of rabbit erythrocytes induced by liposomes were spectrophotometrically determined at 575 nm. RESULTS The three formulations of cucurbitacin B galactosylated liver-targeting liposomes with different adjuvants did not show any hemolytic behavior. The hemolysis percentage of the three formulations were all below 5% by using 10%Triton X-100 as the emulsion-breaker. CONCLUSION Three cucurbitacin B galactosylated liver-targeting liposomes formulations are injectable. The union of macroscopic observation， the optical microscope observation and the spectrophotometry are reliable and feasible to evaluate the hemolytic behavior. 10%Triton X-100 can be used in the hemolysis test as a good emulsion-breaker for liposomes.
关键词
葫芦素B /
半乳糖化肝靶向脂质体 /
溶血 /
常规体外试管法 /
红细胞形态学
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Key words
cucurbitacin B /
galactosylated liver-targeting liposomes /
hemolysis /
macroscopic observation /
erythrocytes morphology /
spectrophotometry
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董晓辉 李乐 石莉 张玲 卢懿 邓意辉.
葫芦素B半乳糖化肝靶向脂质体体外溶血性的研究[J]. 中国药学杂志, 2011, 46(3): 194-198
DONG Xio-hui;LI Le;SHI Li;ZHNG Ling;LU Yi;DENG Yi-hui.
Study on Hemolysis of the Cucurbitacin B Galactosylated Liver-Targeting Liposomes in Vitro[J]. Chinese Pharmaceutical Journal, 2011, 46(3): 194-198
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