目的 应用高效液相色谱（HPLC）方法，建立多维元素片（29）中维生素B₁、维生素C、维生素B₆和烟酰胺的同时测定方法。 方法 采用Alltima C₁₈ 色谱柱（4.6 mm×250 mm， 5 μm），以50 mmol·L-1磷酸二氢铵溶液（磷酸调至pH 3.0）-乙腈（95∶5）为流动相，流速0.5 mL·min-1，检测波长275 nm。 结果 在本方法中，维生素B₁、维生素C、维生素B₆和烟酰胺的线性关系良好，线性相关系数（r）都大于0.999 7；专属性强，4种成分和药品中其他成分以及强制降解产物之间都有良好的分离度；精密度良好，相对标准偏差（RSD）都小于2.10%；回收率良好，回收率在98.82%和100.3%之间；稳定性能满足实验要求，维生素C在4 h内保持稳定；方法学比较显示：维生素C测定值和国标方法测定值的相关性好。结论 该方法准确、简单、快速，可作为多维元素片（29）中维生素B₁、维生素C、维生素B₆和烟酰胺的同时测定。

OBJECTIVE To establish a HPLC method for the simultaneous determination of Vitamin B₁， Vitamin C， Vitamin B₆ and niacinamide in Vitamins with Minerals Tablets（29）. METHODS An Alltima C₁₈ column （4.6 mm×250 mm， 5 μm） was used for the separation with 50 mmol·L-1 ammonium dihydrogen phosphate （adjusted pH to 3.0 with H₃PO₄） and acetonitrile （95∶5） as a mobile phase at a flow rate of 0.5 mL·min-1. The detection wavelength was 275 nm. RESULTS The method showed good linearity for Vitamin B₁， C， B₆ and niacinamide with all correlation coefficients （r） greater than 0.999 7. The specificity study showed satisfactory resolutions between Vitamin B₁， C， B₆， niacinamide， other ingredients and forced degradation products. The precisions were satisfactory with all relative standard deviations （RSD） lower than 2.1%. The spiked recovery for four compounds ranged from 98.82% to 100.3%. The stability were acceptable with a 4 h stable duration for Vitamin C and a method comparison indicated coincident RESULTS for Vitamin C between this method and standard method. CONCLUSION This method is an accurate， fast and simple for the simultaneous determination of Vitamin B₁， C， B₆ and niacinamide in Vitamins with Minerals Tablets（29）.