摘要
目的 探讨肉苁蓉提取物松果菊苷（ECH）延缓衰老的药理作用及其可能的作用机制。方法 小鼠皮下注射10% D-GAL 10 mL·kg-1，每天1次，连续8周，建立亚急性衰老模型。阳性组和ECH各组分别同时灌胃给予维生素E 40 mg·kg-1和ECH 20，40，60 mg·kg-1，每天1次。检测小鼠抗应激能力、血清中IL-2、IL-6含量以及脑组织中一氧化氮（NO）、超氧化物歧化酶（SOD）和丙二醛（MDA）含量。结果 与正常组比较，模型组小鼠抗应激能力下降，血清IL-2含量和脑组织NO、SOD含量均显著下降，IL-6和脑组织MDA含量显著升高。ECH各剂量组和维生素E阴性对照组均能提高衰老小鼠抗应激能力、血清IL-2含量和脑组织NO、SOD含量，降低IL-6和脑组织MDA含量。结论 ECH能够提高衰老小鼠的免疫能力、NO含量，减轻D-GAL诱导衰老小鼠的活性氧自由基损伤，这些可能是其延缓衰老的机制之一。
Abstract
OBJECTIVE To study the mechanism of anti-aging effect of echinacoside (ECH). METHODS Mice were given 10% D-galactose 10 mL·kg-1 sc, once daily for 8 weeks to establish subacute aging mice model. Vitamin E group and ECH groups were given Vitamin E 40 mg·kg-1 ig and ECH 20，40，and 60 mg·kg-1 ig, respectively. The anti-stress ability, contents of interleukin-2 (IL-2) and interleukin-6 (IL-6) in peripheral blood, and contents of SOD, MDA and NO in brain tissue were investigated. RESULTS The anti-stress ability, contents of IL-2, NO and SOD in serum and brain tissue were obviously decreased in the aging model group compared with those in the normal control group. The contents of IL-6 and MDA in brain tissue were significantly increased. CONCLUSION ECH can enhance immune function and reduce the content of NO in brain tissue, thus relieving oxidative damage of D-galactose on aging rats. This may be one of its mechanisms of anti-aging.
关键词
肉苁蓉 /
松果菊苷 /
衰老 /
超氧化物歧化酶
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Key words
Herba Cistanche /
echinacoside /
aging /
SOD
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李媛 宋媛媛 褚春明 张洪泉.
松果菊苷延缓衰老作用机制研究[J]. 中国药学杂志, 2011, 46(14): 1077-1080
LI Yun;SONG Yun-yun;CHU Chun-ming;ZHNG Hong-qun.
Study on the Anti-aging Effect of Echinacoside[J]. Chinese Pharmaceutical Journal, 2011, 46(14): 1077-1080
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脚注
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