摘要
目的 研究3种海绵 Igernella mirabilis， Agelas sp.， Polycarpa aurata的抗菌化学成分。方法 应用活性跟踪的方法，采用硅胶柱色谱，ODS柱色谱和HPLC进行分离纯化，通过核磁共振波谱和高分辨质谱分析进行结构鉴定。结果 从3种海绵Igernella mirabilis， Agelas sp.， Polycarpa aurata各自的CHCl3-MeOH (1∶1)提取物中分离并鉴定了3个抗菌的活性化合物，并鉴定其结构为：halisulfate 7 (1)， agelasine-D (2)和polycarpine(3)。结论 化合物1从海绵Igernella mirabilis首次分离得到，化合物1~3是各自海绵中主要的抗菌化合物， 其中化合物2对新生隐球菌，金黄色葡萄球菌存在良好的抗菌活性，其IC50 / MIC分别为0.76/1.25 mg·L-1， 0.78/1.25 mg·L-1，是很有潜力的抗菌先导化合物。
Abstract
OBJECTIVE To study the antimicrobial chemical constituents of three marine sponges. METHODS By means of bioassay guided isolation，the antimicrobial chemical constituents were isolated and purified by chromatographic techniques with silica gel， ODS column， and preparative HPLC. The structures of the compounds were identified by NMR and HR-ESIMS spectroscopic methods. RESULTS Three antimicrobial compound 1-3 were obtained and elucidated as halisulfate 7 (1)， agelasine-D (2) and polycarpine(3). CONCLUSION Compound 1 was isolated from sponge Igernella mirabilis for the first time. Compound 1-3 were major antimicrobial constituent in marine sponges. Compound 2 inhibited strongly the growth of C. neoformans ATCC 90113 and S. aureus ATCC 29213， their IC50 / MIC were 0.76/1.25 mg·L-1 and 0.78/1.25 mg·L-1 respectively. Compound 2 is a potential leading compound.
关键词
海绵 /
分离 /
结构鉴定 /
抗菌活性
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Key words
marine sponge /
isolation /
structure identification
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徐文晖 梁倩 梁宗锁 李兴从.
3种海绵的抗菌化学成分研究[J]. 中国药学杂志, 2010, 45(5): 332-334
XU Wen-hui;LING Qin;LING Zong-suo;LI Xing-cong.
Antimicrobial Chemical Constituents from Three Marine Sponges[J]. Chinese Pharmaceutical Journal, 2010, 45(5): 332-334
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参考文献
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