目的 研制肝动脉栓塞用吡柔比星-碘油微乳并对其体外抗肿瘤活性进行研究。 方法 采用伪三元相图法研究不同表面活性剂、助表面活性剂和K_m值形成微乳的能力及区域，优选微乳处方，制备吡柔比星-碘油微乳，并对微乳的物理性质、稳定性进行研究；采用MTT法对吡柔比星-碘油微乳体外抗肿瘤活性进行考察。结果 碘化油/大豆磷脂/无水乙醇/水形成的微乳粒径小、黏度低，初步稳定性实验表明，4 ℃下放置药物含量和粒径均无显著变化。MTT实验结果表明，吡柔比星-碘油微乳对人肝癌细胞有明显抑制作用，空白微乳对肝癌细胞HepG-2抑制作用弱。结论 吡柔比星-碘油微乳制备简单，工艺重复性良好，质量稳定，体外抗肿瘤活性较强，为肝动脉栓塞给药剂型的改进提供了依据。

OBJECTIVE To study the preparation of pirarubicin-lipiodol microemulsion for transcatheter arterial chemoembolization and its anti-tumor activity in-vitro.METHODS Pseudo-ternary phase diagrams developed by water titration method were used to select the suitable surfactant，co-surfactantand and their proportion. Physico-chemical property and stability of pirarubicin-lipiodol microemulsion were evaluated. MTT method was used to detect the toxicity of THP microemulsion against human hepatoma cell (HepG-2). RESULTS Pirarubicin-lipiodol microemulsion prepared with Lipiodol/Lipoid S100/ ethyl alcohol/water was with small droplet size and low viscosity. The preliminary stability test showed that the drug content and droplet size of pirarubicin-lipiodol microemulsion were stable at 4 ℃ . MTT assay showed that pirarubicin-lipiodol microemulsion apparently inhibited the proliferation of HepG-2 cell. Blank microemulsion had little cytotoxicity on HepG-2 cell. CONCLUSION The pirarubicin-lipiodol microemulsion is easy to prepare， its quality is stable， and it shows a high anti-tumor activity in vitro， which provide the basis for the optimizing of the dosage form for TACE.