摘要
目的 建立外标法测定乙烷硒啉含量的方法。方法 采用NUCLEODUR C18柱(4.6 mm×250 mm,5 μm),流动相为甲醇-水(0.01%H3PO4)=40∶60,流速1.0 mL·min-1,检测波长320 nm,柱温:25 ℃,外标法测定乙烷硒啉原料药的含量。结果 乙烷硒啉线性范围为10.0~500.0 μg· mL-1,r=0.999 9。乙烷硒啉样品溶液在24 h内稳定,精密度良好,平均回收率为98.64%,RSD=0.40%(n=9)。结论 建立的方法准确、简便、易行,可用于质量控制。
Abstract
OBJECTIVE To establish a HPLC method for the determination of Ethaselen. METHODS A NUCLEODUR C18 column (4.6 mm×250 mm,5 μm) was used with a mobile phase consisting of methanol-water(0.01% phosphoric acid) at a flow rate of 1.0 mL·min-1. The column temperature was 25 ℃ and UV detector wavelength was at 320 nm. RESULTS The calibration curve was linear in the range of 10.0-500.0 μg·mL-1 (r=0.999 9). The average recovery was 98.64% and RSD of 0.40% (n=9). CONCLUSION This method was accurate, simple and suitable for the determination of ethaselen.
关键词
乙烷硒啉 /
含量测定 /
高效液相色谱法
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Key words
ethaselen /
determination /
HPLC
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黄荣华 唐菀晨 刘涛 富戈 曾慧慧 .
RP-HPLC测定乙烷硒啉的含量[J]. 中国药学杂志, 2010, 45(22): 1761-1762
HUNG Rong-hu;TNG Wn-chen;LIU To;FU Ge;ZENG Hui-hui;.
Determination of Content Ethaselen by RP-HPLC[J]. Chinese Pharmaceutical Journal, 2010, 45(22): 1761-1762
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参考文献
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脚注
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