Preliminary Study of the Quality Standards of Essential Oil in the Cultured Artemisia annua
YU Zheng-wen1，2a， WANG Bo-chu1*， YANG Zhan-nan2b， ZHU Lian-cai1
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1.College of Bioengineering， Chongqing University， Chongqing 400044， China; 2. Guizhou Normal University， a. College of Life Science; b. Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou Province， Guiyang 550001， China
OBJECTIVE To establish the quality standards of the cultured Artemisia annua essential oils， and to investigate twenty-one cultured A. annua that collected from Chongqing， China. METHODS The essential oils were extracted with steam distillation method from A. annua leaves and their chemical compositions were analyzed with GC-MS method. RESULTS Seventy-two compounds with high content (≥1.00%) were identified. camphor (2.58%-37.50%)， borneol (2.40%-40.58%)， β-caryophyllene (2.02%-13.10%)， germacrene D(0.00%-10.85%)， caryophyllene oxide (0.00%-10.33%)， spathulenol (0.00%-7.55%)， 2-(4a，8-Dimethyl-1，2，3，4，4a，5，6，7-octahydro-2-naphthalenyl)-2-propen-1-ol(0.00%-5.62%)， cadinol(0.00%-4.83%)， β-selinene (0.00%-3.25%) were obtained widely in essential oils. The total content of the nine compounds was more than 50% in sixteen essential oils， and the highest was up to 85.21%. CONCLUSION In combination our results with the pharmacological activity from the literature， the quality standards were developed as follows： the total content of the above nine compounds in the essential oil of A. annua should not be less than 50.00%.
YU Zheng-wen;WNG o-chu;YNG Zhn-nn;ZHU Lin-ci.
Preliminary Study of the Quality Standards of Essential Oil in the Cultured Artemisia annua [J]. Chinese Pharmaceutical Journal, 2010, 45(2): 98-100
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