目的 建立一种HPLC-UV同时测定清热灵颗粒中5种成分（黄芩苷、连翘苷、汉黄芩苷、黄芩素和汉黄芩素）的含量。方法 采用Agilent Zorbax SB-C18色谱柱（4.6 mm×250 mm，5 μm），以0.05％甲酸-水和0.05％甲酸-乙腈为流动相，梯度洗脱。流速为1.0 mL·min-1，检测波长为274 nm。结果 5个成分的峰面积与浓度的线性关系良好（r2≥0.999 7）；加样回收率（RSD≤3.77％）、精密度（RSD≤0.65％）、重现性（RSD≤2.78％）和稳定性（RSD≤3.19％）均符合有关要求。结论 该方法简单，准确可靠，重现性好，可用于清热灵颗粒质量的控制和评价。
Abstract
OBJECTIVE To establish a HPLC-UV method for the simultaneous determination of five compounds including baicalin, phillyrin, wogonoside, baicalein and wogonin in Qingreling granules. METHODS The separation was performed on an Agilent Zorbax SB-C18 column (4.6 mm×250 mm, 5 μm) with gradient elution. The mobile phases consisted of 0.05% formic acid-acetonitril and 0.05% formic acid-water at a flow rate of 1.0 mL·min-1. The detective wavelength was 274 nm. RESULTS 5 compounds showed good linearity (r2≥0.999 7) in the ranges of tested concentration. The recoveries(RSD≤3.77％), reproducibility(RSD≤0.65％), precision(RSD≤2.78％) and stability (RSD≤3.19％) for 5 compounds were satisfactory. CONCLUSION The method is simple, accurate and reliable. It may be used for the quality control of Qingreling granules.
关键词
清热灵颗粒 /
高效液相色谱法 /
黄芩苷 /
连翘苷 /
汉黄芩苷 /
黄芩素 /
汉黄芩素
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Key words
Qingreling granules /
HPLC /
baicalin /
phillyrin /
wogonoside /
baicalein /
wogonin
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参考文献
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［2］ LIU B, ZHAO L, WU F, et al. Quality control for Qingreling granules . Chin Tradit Pat Med(中成药), 2004, 26 (12): 1016-1018.
［3］ NAKAMURA N, HAYASAKA S, ZHANG X Y, et al. Effects of baicalin, baicalein, and wogonin on interleukin-6 and interleukin-8 expression, and nuclear factor-kappab binding activities induced by interleukin-1beta in human retinal pigment epithelial cell line . Exp Eye Res, 2003, 77(2): 195-202.
［4］ HONG T, JIN G B, CHO S, et al. Evaluation of the anti-inflammatory effect of baicalein on dextran sulfate sodium-induced colitis in mice . Planta Med, 2002, 68 (3): 268-271.
［5］ CHANG W H, CHEN C H, LU F J. Different effects of baicalein, baicalin and wogonin on mitochondrial function, glutathione content and cell cycle progression in human hepatoma cell lines . Planta Med, 2002, 68 (2): 128-132.
［6］ SHIEH D E, LIU L T, LIN C C. Antioxidant and free radical scavenging effects of baicalein, baicalin and wogonin. Anticancer Res, 2000, 20 (5A): 2861-2865.
［7］ LIN C C, SHIEH D E. The anti-inflammatory activity of Scutellaria rivularis extracts and its active components, baicalin, baicalein and wogonin . Am J Chin Med, 1996, 24 (1): 31-36.
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