摘要
目的 对薰衣草不同部位中齐墩果酸和熊果酸进行含量测定。方法 采用反相高效液相色谱法，使用Kromasil C18柱(4.6 mm×250 mm，5 μm)，流动相为甲醇-水-磷酸(90∶10∶0.1)，流速0.8 mL·min-1，检测波长210 nm，柱温25 ℃。结果 齐墩果酸、熊果酸分别在0.50～9.00 μg和0.92～16.56 μg内线性关系良好，回收率（n=9）分别为98.1%（RSD为1.8%)和98.3%（RSD为1.2%)。结论 本方法能够准确测定薰衣草中齐墩果酸和熊果酸的含量。
Abstract
OBJECTIVE To determine oleanolic acid and ursolic acid in Lavandula angustifolia Mill.. METHODS The separation was performed on a Kromasil C18 column (4.6 mm×250 mm，5 μm). The mobile phase consisted of methanol-water-phosphoric acid (90∶10∶0.1). The flow rate was 0.8 mL·min-1 at 25 ℃，and the detection wavelength was set at 210 nm. RESULTS The linear ranges of oleanolic acid and ursolic acid were 0.50 to 9.00 μg and 0.92 to 16.56 μg， respectively. The average recoveries (n=9) for oleanolic acid and ursolic acid were 98.1% (RSD=1.8%) and 98.3%(RSD=1.2%)， respectively. CONCLUSION This method is accurate， reliable and sensitive for the determination of oleanolic acid and ursolic acid in Lavandula angustifolia.
关键词
薰衣草 /
齐墩果酸 /
熊果酸 /
高效液相色谱法 /
光电二极管阵列检测器
{{custom_keyword}} /
Key words
Lavandula angustifolia Mill /
oleanolic acid /
ursolic acid /
HPLC /
photodiode array detector
{{custom_keyword}} /
邹盛勤.
HPLC-PAD法测定薰衣草不同部位中齐墩果酸和熊果酸的含量[J]. 中国药学杂志, 2010, 45(12): 949-951
ZOU Sheng-qin.
Determination of Oleanolic Acid and Ursolic Acid in the Different Parts of Lavandula angustifolia Mill. by HPLC-PAD[J]. Chinese Pharmaceutical Journal, 2010, 45(12): 949-951
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Chinese Pharmacopoeia Commission of Sanitary Ministry of People’s Republic of China. Chinese Pharmacopoeia， Uigur Pharmacopoeia Fascicule(中华人民共和国卫生部药品标准维吾尔药分册)[S].Urumchi： Xinjiang Science and Technology Sanitation Press， 1998：112.
[2] Sanitary Ministry of Xinjiang Uigur Municipality.The Standard of Uigur Medicinal Materials[维吾尔药材标准(上册)] [S]. Urumchi： Xinjiang Science and Technology Sanitation Press， 1991： 394.
[3] ZHANG Q， ZHA L L. Research and application of Lavandula augustifolia[J].Lishizhen Med Mater Med Res(时珍国医国药) ， 2008， 19(6)：1312-1314.
[4] HU X L， ZHAO H， LIU Y F， et al. Study on the chemical components of essential oil extracted in various ways from the lavender by GC-MS[J].Food Sci (食品科学)，2005，(9)：414-416.
[5] ZHANG C L， ZHAO J B， ZHANG C Y， et al. Stydy on the chemical components of Yunnan lavender volatile oil by GC/MS[J]. Henan Sci (河南科学)，1999，17(4):388-391.
[6] WU X， LIU J，YU Z B， et al. Chemical constituents of Lavandula augustifolia[J]. Acta Chim Sin(化学学报)， 2007，35(16) ：137-141.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
PDF(730 KB)
