目的 建立RP-HPLC同时测定豨莶草中奇壬醇与豨莶精醇的含量。方法 色谱柱为Kromasil C₁₈ 柱(4.6 mm×200 mm， 5 μm)，流动相为乙腈-水溶液，梯度洗脱(0~6 min，29.5%乙腈， 6~10 min，29.5%~43.5% 乙腈)，流速为1.0 mL·min^-1，柱温 30 ℃，检测波长 215 nm。结果 奇壬醇在0.015 1~0.302 4 mg·mL^-1内线性关系良好，r=0.999 8，平均回收率为101.5%(RSD=1.7%，n=9)；豨莶精醇在0.002 6~0.052 0 mg·mL^-1内线性关系良好，r=0.999 7，平均回收率为101.7%(RSD=2.0%，n=9)。结论 该方法简便快速，结果准确可靠，重复性良好，适于中药豨莶草中奇壬醇与豨莶精醇的同时测定，并应用本方法比较了不同产地豨莶草药材中奇壬醇与豨莶精醇的含量。

OBJECTIVE To establish a RP-HPLC method for simultaneous determination of kirenol and darutigenol in Herba Siegesbeckiae.METHODS The separation was performed on a Kromasi1 C₁₈ column (4.6 mm×200 mm， 5 μm). Acetonitrile-water was used as the mobile phase with gradient elution(0-6 min， 29.5% acetonitrile， 6-10 min， 29.5%-43.5% acetonitrile). The flow rate was 1.0 mL·min-1 .The column temperature was 30 ℃ and the UV detection wavelength was 215 nm.RESULTS The calibration curves of kirenol were in good linearity in the range of 0.015 1-0.302 4 mg·mL 1 ( r=0.999 8) . The average recovery was 101.5% (RSD=1.7%，n=9). The calibration curves of darutigenol were in good linearity in the range of 0.002 6-0.052 mg·mL-1 (r=0.999 7).The average recovery was 101.7% (RSD=2.0%， n=9). CONCLUSION The method is simple， accurate and sensitive with good reproducibility and could be used for the simultaneous determination of kirenol and darutigenol in Herba Siegesbeckiae. The contents of kirenol and darutigenol in Herba Siegesbeckiae from different places were compared by this method.