目的 N + 注入诱变选育纳豆芽孢杆菌 , 获得溶血栓酶高产菌株，优化发酵工艺。 方法 N+ 注入诱变纳豆芽孢杆菌，在摇瓶中进行发酵工艺优化。 结果 在 N+ 注入剂量为 3.64 × 1015 ions·cm-2 时，正突变率最高，突变株 234 表现出良好的稳定性。最佳发酵培养基和发酵条件为：碳源葡萄糖 , 氮源大豆蛋白胨 , 碳氮比 3 ∶ 1 （ 6% 葡萄糖， 2% 大豆蛋白胨），起始 pH 7, 温度 30 ℃，接种量 8% ，装液量为每 100 mL 为 20 mL 。在最佳发酵工艺下，突变菌株 234 的产酶活性酶活达到 1 380 U·mL-1 。 结论 诱变选育的正突变株性状优良， N+ 注入诱变是一种有效的菌种选育方法。
Abstract
OBJECTIVE To obtain a high producing fibrinolic enzyme mutant of Bacillus nattto and optimize the fermentation condition. METHODS Bacillus nattto was mutated by N+ Implantation and fermentation condition was optimized in a shaking flask. RSEULTS Under the implantation dose of 3.64× 1015 ions·cm-2, the percentage of the positive mutated strain was high. Mutant strain 234 showed excellent genic stability. The optimal culture medium and fermentation condition was as following：carbon source was glucose, nitrogen source was soybean peptone, the ratio of carbon to nitrogen was 3∶1（6% glucose，2% soybean peptone), original pH was 7, temperature was 30 ℃, innoculumon was 8%, seed liquid volume in a 100 mL flask was 20 mL.In the optimum process, the fibrinolic enzyme activity of strain 234 reached to 1 402 U·mL-1. CONCLUSION The positive mutant was excellent and mutation by N+ implantation was useful for breeding strain.
关键词
N+ 注入 /
溶栓酶 /
纳豆芽孢杆菌 /
发酵
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Key words
N+ implantation /
fibronolic enzyme /
Bacillus natto /
fermentation
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参考文献
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（ 收稿日期 ： 2009-04-13 ）
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