目的 研究刮筋板 Excoecaria acerifclia F. Didr. 的化学成分和抗肿瘤活性。 方法 采用硅胶柱色谱， Sephedex LH - 20 分离化学成分 ; 根据波谱数据和理化性质确定化学结构；四甲基偶氮唑蓝（ MTT ） 法测定化合物对人肝癌细胞系 ( human hepatocarcinoma cell line ) HepG2 的 体外抑制作用，用半数抑制浓度 (IC50) 评价其抗肿瘤活性。 结果 分离鉴定 6 个化合物，分别为：秦皮苷（ 1 ）、异落叶松脂醇 9-<>O -β-D- 葡萄糖苷（ 2 ）、 (-)-5 ′ -methoxyisolariciresinol 3a- O-β-<>D-glucopyranoside （ 3 ）、没食子酸（ 4 ）、 isostrictiniin （ 5 ）、 3-<>O- 阿魏酰基奎尼酸甲酯（ 6 ）；化合物 1 ， 2 ， 4 ， 6 对体外培养肿瘤细胞的 IC50 分别为 14.71 ， 26.52 ， 34.91 ， 65.42 μmol·L-1 ； 在测定浓度范围内剂量依赖关系良好。 结论 所有化合物均为首次从该植物中得到；首次研究发现化合物 1 对 HepG 2 具有一定的抗肿瘤活性。
Abstract
OBJECTIVE To investigate the chemical constituents of Excoecaria acerifclia F. Didr. and their antitumor activities. METHODS The constituents of EtOAc-soluble portion were isolated and purified by column chromatography. Their structures were identified by the their physicochemical properties and spectral features. The cytotoxicities of the purified compounds were evaluated by MTT method against human cancer cell lines HepG2. RESULTS Six compounds were isolated and identified as fraxin (1), isolariciresinol 9-O- β-D-glucopyranoside (2), (-)-5′-methoxyisolariciresinol 3a-O-β-D-glucopyranoside (3), gallic acid (4), isostrictiniin (5) and 3-O-feruloylquinic acid methyl ester (6). CONCLUSION All compounds were isolated from this plant for the first time. Compound 1 showed inhibitory activity towards HepG2 with IC50 values of 14.71 μmol·L-1.
关键词
刮筋板 /
化学成分 /
抗肿瘤活性
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Key words
Excoecaria acerifclia F. Didr. /
chemical constituents /
antitumor activities
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参考文献
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（ 收稿日期 ： 2008-12-10 ）
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