目的 考察秦皮香豆素在不同实验条件下的提取与电泳行为，建立其中主要活性成分秦皮苷、秦皮甲素、秦皮乙素的毛细管区带电泳快速定量方法。 方法 采用 50 mmol·L^-1 硼砂溶液为运行缓冲溶液，熔融石英毛细管有效长度 45 cm （ 75 μm × 53.5 cm ），以对羟基苯甲酸丙酯为内标，操作电压 18kV ，柱温 25 ℃，于 210 nm 波长处测定。 结果 在优化的电泳条件下， 3 种秦皮香豆素在 10 min 内完全分离。秦皮苷、秦皮甲素、秦皮乙素的线性范围分别为： 6.0 ～ 96 mg·L^-1 （ <> r =0.999 7 ， <> n =5 ）， 7 ～ 112 mg·L^-1 （ <> r =0.999 6 ， <> n =5 ）和 4.7 ～ 75.2 mg·L^-1 （ <> r =0.999 4 ， <> n =5 ）；平均加样回收率分别为 101.74% ， l00.92% 和 102.44% ，相应的 RSD 分别为 2.18% ， 1 ． 97% ， 2.66%(<>n=9) 。 结论 本实验为秦皮苷、秦皮甲素和秦皮乙素的同时定量提供了一种快速简便的检测方法。

OBJECTIVE To develop a rapid and simple capillary zone electrophoresis method for determining fraxin, esculin and esculetin in Cortex fraxini. METHODS A fused silica capillary (75 μ m × 53.5 cm, with effective length of 45.0 cm) was used. The running buffer was 50 mmol· L^-1 borax solution. Propyl p-hydroxybenzoate was used as an internal standard and detection wavelength was 210nm. Applied voltage was 18kV and Capillary temperature was set at 25 ℃ . RESULTS While all three compounds were completely separated in 10 min, the linear calibration were obtained over the range of 6.0 ～ 96 mg·L ^-1 (<>r=0.999 7, <>n=5) for fraxin ， 7 - 112 mg·L ^-1 （ <> r =0.9996 ， <> n =5 ） for esculin, and 4.7 - 75.2 mg·L ^-1 （ <> r =0.999 4, <>n=5 ） for esculetin. The average recoveries was 101.74 ％ (RSD=2.18%, <>n=9) ， l00.92% (RSD=1.97%, <>n=9) ， 102.44 ％ (RSD=2.66%, <>n=9), respectively. CONCLUSION This method is rapid, accurate and simple for the simultaneous determination of fraxin, esculin and esculetin in Cortex Fraxini.