目的 探讨白附子提取物对人胃癌 SGC-7901 细胞增殖抑制和凋亡诱导作用及其可能机制。 方法 分别采用噻唑蓝（ MTT ）法检测细胞增殖活性、流式细胞仪检测细胞周期及凋亡、光镜观察细胞结构变化、 Hoechst33342/PI 荧光染色检测细胞凋亡、 Western blotting 法检测 caspase-3 、 Bax 和 Bcl-2 蛋白表达。 结果 0.072~7.2 mg · L-1 白附子提取物处理 24 h 后，细胞增殖明显受到抑制，且抑制率随时间延长而增加；光镜下可观察到细胞凋亡形态的改变； Western blotting 结果显示，半胱氨酸蛋白酶（ caspase-3 ）酶原蛋白的激活， Bcl-2 蛋白表达的降低和 Bax 蛋白表达上调。 结论 白附子提取物通过影响 SGC-7901 细胞的增殖、周期和凋亡相关蛋白的表达发挥凋亡诱导作用。
Abstract
OBJECTIVE To explore the anti-proliferation and apoptosis-induced effects of Rhizoma Typhonii extracts on SGC-7901 cells, and possible mechanisms. METHODS SGC-7901 cell proliferative activity was measured by MTT assay. Cell cycle and apoptosis were analyzed by flow cytometry（FCM）. Cell apoptosis was observed by Hoechst33342/PI fluorescent staining. The protein levels of caspase-3, Bax and Bcl-2 were detected by Western blotting. RESULTS After exposure to Rhizoma Typhonii extracts at concentrations from 0.072 to 7.2 mg·L-1 for 24 h,cellular proliferation was inhibited with the increase of the concentration and the time. The significant apoptotic morphology changes were observed. Western blotting showed the activity of the caspase-3, increase of expression of bax protein, and decrease of expression of bcl-2 protien. CONCLUSION Rhizoma Typhonii extracts induce apoptosis by up-regulation of bax protein expression and down-regulation of bcl-2 protein in SGC-7901 cells.
关键词
白附子 /
凋亡 /
半胱氨酸蛋白酶 -3 /
B 细胞淋巴瘤基因 -2 /
Bax
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Key words
Rhizoma Typhonii /
apoptosis /
caspase-3 /
Bcl-2 /
Bax
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参考文献
[1] SHAN B N, ZHANG J Y, LI Q X, <>et al. Human T cell and monocyte modulating activity of Rhizoma Tvnhonii <>in vitro[J]. <>Chin J Integy Tradit West Med （中国中西医结合杂志） , 2001, 21(10):768-772.
[2] LI Y L, ZHONG L, LIANG E H. The experimental studies of six kinds of Chinese medicines inhibiting tyrosinase activity [J]. <>Lishizhen Med Mat Med Res （时珍国医国药） , 2002, 13(3):129-131.
[3] WU G J, ZHANG Z W, LI H R, <>et al. Antibacterial effects of Chinese herbal medicines on six pathogenic bacteriafrom dairy cows mastitis [J]. <>J Beijing Univ Agr （北京农学院学报） , 2003, 18(3):195-198.
[4] JIANG D ， WANG G L. Study on antibacterial activity of twenty-two kinds of Chinese medicines[J]. <>Liaoning High Voc Tech Ins J （辽宁高职学报） ,2003,5(4):140-141.
[5] HU C X, ZHU J, SUN X J. Study on chemical composition and pharmacological effects of <>Typhonium gigantewm Engl. [J]. <>Agriculture Technol （农业与技术） , 2007, 27(2):50-54.
[6] SUN S F,ZENG Y,ZHAO W C. Empirical studies of inhibition malignant tumor by giant typhonium herb[J]. <>Shaanxi J Tradit Chin Med J( 陕西中医 ) ， 1999 ， 20 （ 2 ）： 94 .
[7] WANG Q S, NI H, WANG J, <>et al. The inhibition effects of <>Typhonium giganteum Engl. on hepatocarcinoma cell[J]. <>Chin J Cell Biology J （细胞生物学杂志）， 2003 ， 25 （ 3 ）： 185-188.
[8] WANG Y . Studies on anti-tumor activity and mechanism of Giant Typhoniumh Rhizom[D]. Changchun: Jilin University, 2002.
[9] SHANG L, WANG L, SUN H L, <>et al. Protective effects of oxymatrine on myocyte apoptosis in rats with acute myocardial ischaania[J]. <>Chin Pharm J （中国药学杂志） , 2007 ， 42 （ 7 ）： 501-504.
[10] KRAJEW SKI S, KRAJEW SKA M, SHABAIK A, <>et al. Immunohistochemical determination of <>in vivo distribution of Bax, adominant inhibitor of Bcl-2 [J]. <>Am J Pathol, 1994, 145 (6):1323-1336.
（收稿日期： 2008-08-03 ）
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