红车轴草总黄酮体外抗氧化作用的研究

刘宝剑;郭延生 ;曲亚玲 ;刁鹏飞 ;魏彦明

中国药学杂志 ›› 2009, Vol. 44 ›› Issue (16) : 1234-1236.

PDF(2356 KB)
PDF(2356 KB)
中国药学杂志 ›› 2009, Vol. 44 ›› Issue (16) : 1234-1236.
论文

红车轴草总黄酮体外抗氧化作用的研究

  • 刘宝剑 1,2 ,郭延生 1 ,曲亚玲 1 ,刁鹏飞 1 ,魏彦明 1*
作者信息 +

Study on Antioxidation of Total Flavonoids Red Clover <>in Vitro

  • LIU Bao-jian1 , 2 , GUO Yan-sheng1 , QU Ya-ling1 , DIAO Peng-fei1 , WEI Yan-ming1*
Author information +
文章历史 +

摘要

目的 探讨红车轴草总黄酮体外抗氧化作用。 方法 采用分光光度法测定 H2O2 诱导红细胞溶血作用,用 2- 硫代巴比妥酸法测定小鼠红细胞膜脂质及肝组织匀浆脂质过氧化产物丙二醛( MDA )含量。 结果 红车轴草总黄酮在 0.2~1.6 mg·L-1 内能抑制 H2O2 诱导红细胞溶血和红细胞膜脂质过氧化作用, IC50 分别为 0.93 及 0.92 mg·L-1 ,均呈较好的量效关系;红车轴草总黄酮在 4~32 mg·L-1 内能抑制肝组织脂质过氧化产物的形成, IC50 为 17.5 mg·L-1 。 结论 在一定浓度范围内红车轴草总黄酮体外具有明显的抗氧化作用。

Abstract

OBJECTIVE To study the anti-oxidation of the flavonoids in the red clover in vitro. METHODS Spectrophotometry was used to measure the hemolysis of the erythrocytic induced by H2O2 and TBA colorimetry to study the content of lipid peroxidation products (MDA) in the erythrocytes and the liver homogenates of mice. RESULTS The results indicated that the flavonoids of the red clover at the concentration of 0.2-1.6 mg·L-1 significantly inhibited the erythrocytic hemolysis induced by hydrogen peroxide and the lipid peroxidation of erythrocytic membranes.50% inhibitory concentrations (IC50) were 0.93 and 0.92 mg·L-1 respectively, showing a appropriate concentration-effect relationship. And they obviously prevented the formation of MDA lipid peroxidation in homogenates of mouse liver at the concentration of 4-32 mg·L-1, with IC50 of 17.5 mg·L-1. CONCLUSION The flavonoids of the red clover have a significant antioxidation effect within a range of certain concentration in vitro.

关键词

红车轴草总黄酮 / 抗氧化 / 脂质过氧化 / 红细胞溶血

Key words

red clover total flavonoids / antioxidation / lipid peroxidation / erythrocytic hemolysis

引用本文

导出引用
刘宝剑;郭延生 ;曲亚玲 ;刁鹏飞 ;魏彦明 . 红车轴草总黄酮体外抗氧化作用的研究[J]. 中国药学杂志, 2009, 44(16): 1234-1236
LIU o-jin;GUO Yn-sheng;QU Y-ling;DIO Peng-fei;WEI Yn-ming . Study on Antioxidation of Total Flavonoids Red Clover <>in Vitro[J]. Chinese Pharmaceutical Journal, 2009, 44(16): 1234-1236

参考文献


[1] HU X X , SUN X D . <> Forage Grasses from China (国产牧草植物) [M] . Beijing : Science & Technology Press , 1955 : 68 - 70 .
[2] CHEN M J , JIA S X . <> China Feeding Plants (中国饲用植物) [M] . Bejing : Chinese Agricultural Publishing House , 2002 : 654 - 655 .
[3] LIU Y , LIU S H , WANG P . Advance in researches on red clover[J]. <>Chin Tradit Herb Drugs (中草药), 2007 , 38 ( 5 ): 801 - 804 .
[4] LI C M , XIANG Y , ZHAI L , <> et al . Study on refining process of red clover total isoflavone[J] . <> Chin Tradit Herb Drugs (中草药), 2004 , 35 ( 10 ): 1135 - 1136 .
[5] ZHAO Y T , GUO X M , LI F Z . Anti-oxidative activity of polysaccharide from Rosa laevigata Michx[J]. <>J Biol (生物学杂志), 2003 , 20 ( 2 ): 23 - 24 .
[6] GAO X , ZHANG Z M , XU A X , <> et al . Antioxidation of ethanolic extract from saussurea laniceps on tissues and erythrocytes in rats[J]. <>Chin Pharm J (中国药学杂志), 2005 , 40 ( 14 ): 1062-1065 .
[7] YAN Y C , LOU X E , JIANG H D . Experimental studies on the anti-oxidation effects of water extract from <>Chrysanthemum indicum L.[J] . <> Chin J Mod Appl Pharm (中国现代应用药学杂志), 1999 , 16(6) : 16 - 18 .
[8] WANG J H , ZHANG M , GAN L , <> et al . Protective effect of polysaccharide-1 from <>Fructus lycii on mice liver mitochondria injury induced by hydroxyl radicals[J] . <> Chin Pharm J (中国药学杂志), 2001 , 36(10) : 669 - 672 .
[9] WU L J . <> Traditional Chinese Medicinal Chemistry (中药化学) [M]. Beijing : China Medical Sci-Tech Publishing House , 2001 : 110 - 111 .
[10] LIU L , JIN H . Research developments on anti-oxidation of soybean isoflavone[J] . <> Chin J Radiol Med Prot (中华放射医学与防护杂志), 2003 , 23(2) : 132 - 134 .
[11] ZHENG R L . <> Free Radical Biology (自由基生物学) [M]. Beijing: Higher Education Press , 1992 : 14 -1 6 .
[12] YANG J X , YANG C , QIU J , <> et al <> In vitro antioxidant properties of forsythia suspense leaves flavonoids[J] . <> Nat Prod Res Dev (天然产物研究与开发), 2007 , 19(1) : 97 - 100 .
[13] ZHU Y J , ZHANG Y , WANG C G , <> et al . Study on the anti-oxidizing action of flavonoids extracted from red clover[J] . <> Food Sci Technol (食品科技), 2006 , 31(4) : 78 - 81 .
( 收稿日期 : 2008-08-05 )




PDF(2356 KB)

Accesses

Citation

Detail

段落导航
相关文章

/