摘要
目的观察E-selectin及P-selectin在羟乙葛根素保护大鼠局灶性脑缺血再灌注损伤中的作用。方法线栓法制备大鼠局灶性脑缺血再灌注损伤模型,大鼠脑缺血2 h再灌注24 h后,采用Western blot及RT-PCR法观察大鼠脑组织E-selectin及P-selectin的蛋白及mRNA表达情况。结果羟乙葛根素可明显降低缺血区脑组织E-selectin及P-selectin的蛋白及mRNA表达。结论羟乙葛根素可能通过降低缺血区脑组织黏附分子表达发挥脑保护作用。
Abstract
OBJECTIVE To observe the effects of hydroxyethylpuerarin on expression of E-selectin and P-selectin in rats with focal cerebral ischemia-reperfusion injury and explore its action mechanism.METHODS Rats were subjected to cerebral ischemia-reperfusion injury by middle cerebral artery occlusion(MCAO).After a 2 h ischemia and 24 h reperfusion,the protein and mRNA expression of E-selectin and P-selectin were evaluated by western blot and RT-PCR analysis.RESULTS Hydroxyethylpuerarin significantly reduced the expression of E-selectin and P-selectin in ischemic brain tissue induced by cerebral ischemia-reperfusion.CONCLUSION Hydroxyethylpuerarin may attenuate brain ischemia injury by down-regulating the expression of adhesion molecules in ischemic brain tissues.
关键词
羟乙葛根素 /
脑缺血再灌注损伤 /
E-selectin /
P-selectin
{{custom_keyword}} /
Key words
hydroxyethylpuerarin /
cerebral ischemia-reperfusion injury /
E-selectin /
P-selectin
{{custom_keyword}} /
娄海燕;魏欣冰;刘慧青;王姿颖;张岫美.
羟乙葛根素对大鼠局灶性脑缺血再灌注损伤后脑组织E-selectin及P-selectin表达的影响[J]. 中国药学杂志, 2008, 43(23): 1788-1791
LOU Hi-yn;WEI Xin-ing;LIU Hui-qing;WNG Zi-ying;ZHNG Xiu-mei.
Effect of Hydroxyethylpuerarin on Expression of E-selectin and P-selectin in Rats with Focal Cerebral Ischemia-Reperfusion Injury [J]. Chinese Pharmaceutical Journal, 2008, 43(23): 1788-1791
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] JEAN W C，SPELLMAN S R，NUSSBAUM E S，et al. Reperfusion injury after focal cerebral ischemia： the role of inflammation and the therapeutic horizon[J] . J Neurosurg，1998，43(6)：1382-1397.
[2] OKADA Y，COPELAND B R，MORI E，et al. P-selectin and intercellular adhesion molecule-1 expression after focal brain ischemia and reperfusion[J] .Stroke，1994，25(1)： 202-211.
[3] SUZUKI H，ABE K，TOJO S，et al. Expressions of P-selectin and HSP72-like immunoreactivities in rat brain after transient middle cerebral artery occlusion[J] .Brain Res，1997，759(2)： 321-329.
[4] PANTONI L，SARTI C，INZITARI D. Cytokines and cell adh- esion molecules in cerebral ischemia： experimental bases and therapeutic perspectives[J] . Arterioscler Thromb Vasc Biol，1998，18：503-513.
[5] WANG Z Y，WEI X B，CHEN L，et al. Neuroprotective effects of hydroxyethylpuerarin against focal cerebral ischemia-reperfusion in rats[J] . Chin J Physiol（中国生理学报），2007，50(5)：1-6.
[6] LOU H Y，ZHANG X M，WEI X B，et al. Anti- inflammatory effect of hydroxyethylpuerarin on focal brain ischemia/reperfusion injury in rats[J] . Chin J Physiol（中国生理学报），2004，47(4)：203-207.
[7] WANG Z Y，ZHANG X M，WEI X B，et al. Effects of hydroxy- ethylpuerarinon on NO level and NOS activity after focal cerebral ischemia-reperfusion injury in rats[J] .Chin Pharm J（中国药学杂志），2006，41（2）：112-114.
[8] LONGA E Z，WEINSTEIN P R，CARLSON S，et al. Reversible middle cerebral artery occlusion without craniectomy in the rats[J] . Stroke，1989，20：84-91.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
