摘要
目的观察海康灵对SH-SY5Y神经细胞凋亡、细胞内线粒体膜电位和钙离子的影响。方法采用血清药理学方法,用流式细胞仪观察海康灵对过氧化氢(H2O2)损伤的SH-SY5Y神经细胞凋亡、细胞线粒体膜电位和钙离子的影响。结果与模型组比较,海康灵含药血清能明显抑制H2O2引起的SH-SY5Y神经细胞的凋亡,稳定线粒体膜电位,抑制钙离子内流。结论以上结果提示,海康灵可通过抑制胞浆钙离子升高,进而稳定线粒体膜电位,从而发挥其抑制神经细胞凋亡的作用。
Abstract
OBJECTIVE To observe the effect of Haikangling in serum on apoptosis,mitochondria membrane potential and Ca2+ of neuroblastoma SH-SY5Y cells.METHODS The effects of Haikangling in serum on apoptosis,mitochondria membrane potential and Ca2+ of SH-SY5Y cells impaired by hydogen peroxide(H2O2)cultured in vitro were measured by flow cytometry.RESULTS Compared with the model group,Haikangling obviously restrained the apoptosis,Ca2+) inflow,and stabilized the mitochondria membrane potential of SH-SY5Y cells.CONCLUSION Anti-dementia effects of Haikangling were related to restraining the apoptosis,stabilizing mitochondria membrane potential and restraining Ca2+ inflow of neurocyte.
关键词
海康灵 /
过氧化氢 /
凋亡 /
线粒体膜电位 /
钙离子
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Key words
Haikangling /
H2O2 /
apoptosis /
mitochondria membrane potential /
Ca2+
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孙向红;孙伟;李静;耿美玉.
中药复方海康灵含药血清对过氧化氢诱导的神经细胞凋亡的影响[J]. 中国药学杂志, 2008, 43(23): 1781-1784
SUN Xing-hong;SUN Wei;LI Jing;GENG Mei-yu.
Effects of Haikangling in Serum on Cultured Neuroblastoma Apoptosis Induced by Hydrogen Peroxide [J]. Chinese Pharmaceutical Journal, 2008, 43(23): 1781-1784
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脚注
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