OBJECTIVE To study the effect of different combinations and concentrations of plant growth regulators on proliferation of axillary buds of Anoectochilus roxburghii.And to observe the course of growth and development of buds and formation of complete plantlets.METHODS Shoot tips and nodal segments were used for axillary shoot proliferation on MS basal medium supplemented with nine kinds of combinations of cytokinin(1 mg·L-1) and auxin(0.5 mg·L-1).Some growth index,such as the course of buds formation and development,buds proliferation,height of buds and shoots,were observed routinely.The effect of different concentration of plant growth regulators on axillary buds proliferation of A.roxburghii was carried out based on screening the suitable regulator combination.RESULTS Axillary buds induction on BA+auxin media was higher than that on MS,KT+auxin and ZT+auxin media.There are usally 2-4 level lateral buds ramifications in media containing BA,higher than other media.The number of buds proliferation per nodal segment and shoot tip induced on BA+NAA medium were maximum,which were 3.62 times and 3.56 times as control,respectively.Multiple buds induction of nodal segments was the highest(14 clusters) on BA+IBA medium.Buds growing on BA+auxin media were robust,its intersegments were shorten and roots were short and thick.The combination of BA 2.0 mg·L-1+NAA 2.0 mg·L-1 generated the good effect on axillary buds proliferation of A.roxburghii,which was 5.29 times as control.Buds(2-2.5 cm long) were cut and transfered into MS medium.Complete plantlets were formed within 25 d.CONCLUSION Different combinations of plant growth regulators with specific concentration have different effects on buds induction,while cytokinin has a major effect.Combinations of BA+NAA and BA+IBA are the optimum media for micropropagation of A.roxburghii.Compared with BA,NAA showed a major effect on the number of buds proliferation concentration.
MENG Zhi-xi;GUO Shun-xing;YU Xue-mei;CHEN Xio-mei;XIO Pei-gen.
Effect of Plant Growth Regulator on Proliferation of Axillary Buds of Anoectochilus roxburghii[J]. Chinese Pharmaceutical Journal, 2008, 43(23): 1777-1780
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