摘要
目的建立反相高效液相色谱法同时测定糖克软胶囊中5种木脂素类(五味子醇甲、五味子醇乙、当归酰戈米辛H、五味子甲素、五味子乙素)成分含量的方法。方法色谱条件:Agilent Extend C18柱;流动相为甲醇-2%四氢呋喃水溶液,线性梯度洗脱,流速0.8 mL·min-1;柱温50℃,检测波长254 nm。结果5种木脂素类化合物在实验浓度内线性关系良好,五味子醇甲、五味子醇乙、当归酰戈米辛H、五味子甲素和五味子乙素的线性范围分别为15.8～1 575,5.76～576,4.65～465,3.61～361,10.6～1 057 mg·L-1,相关系数均大于0.999 0。平均加样回收率在100.2%～103.5%之间。5种木脂素类化合物含量重复性试验RSD小于1.98%。结论本方法简单、灵敏、重复性好,可用于糖克软胶囊质量控制。
Abstract
OBJECTIVE To develop a high performance liquid chromatography method for the simultaneous determination of five major lignans(schisandrin,gomisin A,angeloylgomisin H,deoxyschizandrin and γ-schisandrin) in Tangke capsule.METHODS Chromatographic separation was performed on an Agilent Extend C18 column.The mobile phase was composed of methanol and 2% tetrahydrofuran-water at a linear gradient elution.The column temperature was maintained at 50 ℃.Detection wavelength was 254 nm,and flow rate was 0.8 mL·min-1.RESULTS Good linear relationships of five lignans were provided over investigated concentration ranges.The linear ranges of schisandrin,gomisin A,angeloylgomisin H,deoxyschizandrin and γ-schisandrin were 15.8～1 575,5.76～576,4.65～465,3.61～361,10.6～1 057 mg·L-1,respectively.The values of correlation coefficient were higher than 0.999 for all the analytes.The average recoveries of the five components ranged from 100.2% to 103.5%.Repeatability experiments showed that relative standard deviation(RSD) values of their contents were less than 1.98%.CONCLUSION The developed method is simple,sensitive and repeatable.The method can be used for quality control of Tangke capsule.
关键词
糖克软胶囊 /
五味子 /
木脂素 /
高效液相色谱法
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Key words
Tangke capsule /
Schisandra chinensis /
lignans /
HPLC
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陈颖;邵青;瞿海斌.
HPLC同时测定糖克软胶囊中5种木脂素类成分的含量[J]. 中国药学杂志, 2008, 43(22): 1751-1754
CHEN Ying-jie;SHO Qing;QU Hi-in.
Simultaneous Determination of Five Lignan Compounds in Tangke Capsules by HPLC [J]. Chinese Pharmaceutical Journal, 2008, 43(22): 1751-1754
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参考文献
[1] CHAI K F，YANG M H，YANG S P. The research of Tangke Capsule affects blood sugar of different experimental animal models[J] . Chin Arch J Tradit Chin Med（中医药学刊），2005，23(4)：589-591.
[2] CHAI K F，YANG S P，YANG M H. The research of blood sugar decline of Tangke Capsule[J] . Chin J Tradit Med Sci Technol（中国中医药科技），2006，13(1)：22-23.
[3] CHAI K F，ZHOU M，YANG M H. The research of Tangke Capsule affects diabetes and high sticky blood[J] . Chin J Exp Tradit Med Form (中国实验方剂学杂志)，2006，12(1)：51-52.
[4] CHEN A J，LI C H，GAO W H，et al. Separation and deter- mination of active components in Schisandra chinensis Baill. And its medicinal preparations by non-aqueous capillary electrophoresis[J] . Biomed Chromatogr，2005，19(7)：481-487.
[5] YIN F Z，LU T L，LI L. Comparison of γ-schisandrin contents of Fructus schisandrae of different origins in Heilongjiang province[J] . Acta Univ Tradit Med Sin Pharmacol Shanghai（上海中医药大学学报），2005，19(1)：42-43.
[6] LU T L，YIN F Z，LI L，et al. Determination of contents of schisandrin and γ- schizandrin in Schisandra chinensis (Turcz. ) Baill. By RP-HPLC[J] . Chin Trad Patent Med（中成药），2006，28(8)：1210-1212.
[7] YANG C J，XIONG Z L，YANG J，et al. Determination of contents of deoxyschizandrin and γ- schizandrin in Schisandra chinensis(Turcz. ) Baill. By RP-HPLC[J] . J Shenyang Pharm Univ（沈阳药科大学学报），2006，23(10)：660-663.
[8] WEI D H，SONG Z M，CHEN S X，et al. Determination of content of deoxyschizandrin and γ- schizandrin in the different processed products of Fructus schisandrae Chinensis by HPLC[J] . Prog Mod Biom（现代生物医学进展），2006，6(6)：20-21.
[9] LI J H，FEN W H，KANG C，et al. Determination of content of schizandrin in commercial Wuweizi by HPLC[J] . Chin J Inf on Tradit Chin Med（中国中医药信息杂志），2001，8(12)：31，37.
[10] GE H Q，JIA T Z. The measure of the schizandrol A contents of Frustus schisandrae of different origins in Liaoning province[J] . Chin Archives Tradit Chin Med（中华中医药学刊），2007，25(2)：319-320.
[11] GUO C M. Quantitative analysis of γ- schizandrin in Schisandra chinensis Baill of different producing area[J] . Heilongjiang Med J（黑龙江医药学），2003，16(1)：6-7.
[12] GUO L Q，ZHANG P，HUANG L L，et al. Research progress of the pharmacology of Schisandra chinesis[J] . J Chin Med Pharmacol（中医药学报），2006，34(4)：51-53.
[13] ZHANG M H，CHEN H，LI L Z，et al. Deoxyschizandrin and schizandrin protect liver against carbon tetrachloride induced damage[J] . Med J Chin PAPF（武警医学），2002，13(7)：395-396.
[14] SIU-PO I P，KAM-MING K O. The crucial antioxidant action of schisandrin B in protecting against carbon tetrachloride hepatotoxicity in mice：A comparative study with butylated hydroxytoluene[J] . Biomed Chromatogr，1996，52(11)：1687-1693.
[15] LI X G，GAO Q，WEN W，et al. The effective position and research progress of the pharmacology of Schisandra chinesis[J] . J Chin Med Mater(中药材)，2005，28(2)：156-159.
[16] SHEN B C，ZHAO J F，HU W，et al. Pharmacological activities of lignans in kadsura and clinical application of kadsara[J] . Acad J Kunming Med Coll(昆明医学院学报)，2005，26(1)：102-104.
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脚注
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基金
浙江省科技计划重大科技专项资助项目(2006C13011)
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