Effection of Daidzein on Cultured Vascular Smooth Muscle Cells and the Expression of TSC-36 of Cells
SUN Hai-yan2，WANG Lü-ya1*，Lü Shu-zheng1，LIU Shu1
Author information+
1.Department of Atherosclerosis，Beijing Anzhen Hospital，Capital University of Medical Sciences，Beijing 100029，China； 2. Department of Cardiology，Third Affiliated Hospital of Xinxiang Medical College，Xinxiang 453003，China
OBJECTIVE To observe the inhibition effect of daidzein on cultured vascular smooth muscle cells(VSMCs)and the expression of transforming growth factor beta-stimulated clone 36(TSC-36).METHODS Human umbilical artery smooth muscle cells were cultured and were divided into blank control group and daidzein treatment groups in different concentration.Cellular activity was estimated by MTT.Competitive PCR quantitaly analyzed the expression of TSC-36 in cells.RESULTS MTT showed that cellular activity ratio in daidzein group was effectively decreased compared with control group(P<0.05).Competitive PCR showed that gene contents in daidzein group were significant increased compared with control group(P<0.01).CONCLUSION Daidzein can inhibit the proliferation of cultural VSMCs and can upregulate the expression of TSC-36.
SUN Hi-yn;WNG Lü-y;Lü Shu-zheng;LIU Shu.
Effection of Daidzein on Cultured Vascular Smooth Muscle Cells and the Expression of TSC-36 of Cells [J]. Chinese Pharmaceutical Journal, 2008, 43(22): 1706-1709
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] HWANG J，WANG J，MORAZZONI P，et al. The phytoestrogen equol increases nitric oxide availability by inhibiting superoxide production： an antioxidant mechanism for cell-mediated LDL modification[J] . Free Radic Biol Med，2003，34(10)：1271-1282.
[2] SQUADRITO F，ALTAVILLA D，MORABITO N，et al. The effect of the phytoestrogen genistein on plasma nitric oxide concentrations，endothelin-1 levels and endothelium dependent vasodilation in postmenopausal women[J] . Atherosclerosis，2002，163(2)：339-347.
[3] HAN J，WANG W，WANG L，et al. Effect of puerarin and daidzein on proliferating vascular ooth muscle cells[J] . China J Chin Mater Med(中国中药杂志)，2004，29（5）：437-440.
[4] LIU S，WANG L，WANG W，et al. TSC-36/FRP inhibits vascu- lar smooth muscle cell proliferation and migration[J] . Exp Mol Pathol，2006，80(2)：132-140.
[5] SHIBANUMA M，MASHIMO J，MITA A，et al．Cloning from a mouse osteoblastic cell line of a set of transforming-growth-factor-β1-regulated genes，one of which seems to encode a follistatin-related polypeptide[J] . Eur J Biochem，1993，217(1)：13-19.
[6] ZWIJSEN A，BLOCKX H，VAN ARNHEM W，et al. Character- ization of a rat C6 glioma-secreted follistatin-related protein(FRP) cloning and sequence of the human homologue[J] . Eur J Biochem，1994，225(3)：937-946.
[7] AMTHOR H，CONNOLLY D，PATEL K，et al. The expression and regulation of follistatin and a follistatin-like gene during avian somite compartmentalization and myogenesis[J] . Development Biol，1996，178(2)：343-362.
[8] SUMIMOTO K，KURISAKI A，YAMAKAWA N，et al. Expression of a TGF-beta1 inducible gene，TSC-36，causes growth inhibition in human lung cancer cell lines[J] . Cancer Lett，2000，155(1)：37-46.
[9] OHASHI T，SATO S，YOSHIKI A，et al. TSC-36 (follistatinrel-ated polypeptide) gene expression in estrogen receptor positive osteoblastic cell line，CDO7[J] . Calcif Tissue Int，1997，61(5)：400-403.
[10] KAWABATA D，TANAKA M，FUJII T，et al. Ameliorative effects of follistatin-related protein/TSC-36/FSTL1 on joint inflammation in a mouse model of arthritis[J] . Arthritis Rheum，2004，50(2)：660-668.
