摘要
目的对菊科橐吾属植物阿勒泰橐吾(Ligularia altaica DC.)的萜类成分进行研究并进行抗白血病细胞K562的活性筛选。方法采用硅胶、凝胶柱色谱和重结晶等技术进行分离纯化,根据理化性质和波谱数据并参考文献确定化合物结构,采用MTT法进行抗K562细胞增殖的活性筛选。结果分离鉴定了9个倍半萜化合物:furaneremophilan-14β,6α-olide(1),8β-hydroxyl-7(11)-eremophilen-12,8α-olide(2),7(11)-eremophilen-12,8α-olide(3),8β-hydroxyl-eremophil-7(11)-en-12,8α(14β,6α)-diolide(4),6β-hydroxyl-7(11)-eremophilen-12,8α-olide(5),6β,8β-dihydroxyl-7(11)-en-12β,8α-olide(6),7-acetyl-6(7)-en-noreremophil-13β-carboxylic acid(7),8β-hydroeremophil-7(11)-eremophilen-12,8α(14β,6α)-diolide(8)和2-hydroxyl-plary phillide(9)。结论以上化合物均为首次从该种植物中分离得到,eremophilane型降倍半萜化合物7和9对K562细胞生长显示弱的抑制活性。
Abstract
OBJECTIVE To study the sesquiterpenes of the underground part of Ligularia altaica DC.and their cytotoxicity activity against K562 cell lines.METHODS Nine eremophilane sesquiterpenes were obtained as :furaneremophilan-14β,6α-olide(1),8β-hydroxyl-7(11)-eremophilen-12,8α-olide(2),7(11)-eremophilen-12,8α-olide(3),8β-hydroxyl-eremophil-7(11)-en-12,8α(14β,6α)-diolide(4),6β-hydroxyl-7(11)-eremophilen-12,8α-olide(5),6β,8β-dihydroxyl-7(11)-eremophilen-12,8α-olide(6),7-acetyl-6(7)-en-noreremophil-13β-carboxylic acid(7),8β-hydroeremophil-7(11)-en-12β,8α(14β,6α)-diolide(8) and 2-hydroxyl-plary phillide(9).CONCLUSION All compounds were obtained from this plant firstly,compound 7 and 9 showed relative weak inhibitory activities against K562 cell lines with IC50 as 65.27 and 54.71 mg·L-1,respectively.
关键词
菊科 /
阿勒泰橐吾 /
eremophilane型倍半萜
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Key words
Composetae /
Ligularia altaica DC. /
eremophilane sesquiterpenes
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张朝凤;王琼;张勉.
阿勒泰橐吾的倍半萜类成分研究[J]. 中国药学杂志, 2008, 43(22): 1697-1699
ZHNG Cho-feng;WNG Qiong;ZHNG Min.
Study on Sesquiterpene Components of Ligularia altaica DC. [J]. Chinese Pharmaceutical Journal, 2008, 43(22): 1697-1699
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参考文献
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脚注
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基金
国家自然科学基金资助项目(30270157)
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